Interplay between 5-HT2C and 5-HT1A receptors in the dorsal periaqueductal gray in the modulation of fear-induced antinociception in mice

Autor(es)

Baptista-de-Souza, Daniela ^{[1, 2, 3]} ; Pelarin, Vinicius ^{[1, 3]} ; Canto-de-Souza, Lucas ^{[4, 2]} ; Nunes-de-Souza, Ricardo Luiz ^{[1, 4, 2]} ; Canto-de-Souza, Azair ^{[1, 2, 3, 5]}

Afiliação do(s) autor(es)

^[1] Joint Grad Program Physiol Sci UFSCar UNESP, BR-13565905 Sao Carlos, SP - Brazil

^[2] Inst Neurosci & Behav, Av Cafe 2-450, BR-14050220 Ribeirao Preto, SP - Brazil

^[3] Fed Univ Sao Carlos UFSCar, Dept Psychol, BR-13565905 Sao Carlos, SP - Brazil

^[4] Univ Estadual Paulista UNESP, Sch Pharmaceut Sci, Lab Pharmacol, BR-14800903 Araraquara, SP - Brazil

^[5] Grad Program Psychol UFSCar, Rod Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Neuropharmacology; v. 140, p. 100-106, SEP 15 2018.
• Citações Web of Science: 1

Resumo

The confinement of rodents to the open arm of the elevated-plus maze provokes antinociception (OAA). As a type of defensive reaction, the OAA has been investigated through systemic and intramesencephalic (e.g., dorsal portion of the periaqueductal gray - dPAG) injections of anxiolytic-like drugs {[}e.g., serotonergic (5-HT) receptor agonists or antagonists]. Here we investigated the effects of (i) intra-dPAG injections of a 5HT(2C) receptor agonist (MK-212; 0.21 or 0.63 nmol) and antagonist (SB 242084; 0.01, 0.1 or 1.0 nmol); (ii) combined injections of SB 242084 and MK-212 into the dPAG; (iii) combined injections of SB 242084 with 8-OHDPAT (10 nmol) into the dPAG on the OAA in male Swiss mice. Nociception was assessed with the writhing test induced by acetic acid injection. Results showed that (i) intra-dPAG injection of MK-212 (0.63 nmol) increased the OAA; (ii) intra-dPAG SB 242084 (1.0 nmol) prevented the OAA; (iii) SB 242084 (0.1 nmol, a dose devoid of intrinsic effect on nociception) blocked the OAA enhancement provoked by MK-212 and enabled 8-OH-DPAT to prevent the OAA. These results suggest that OAA is mediated by 5-HT2C receptors within the dPAG. Intra-dPAG SB242084 administration provoked similar results on the effects produced by MK-212 and 8-OH-DPAT on OAA. In addition, the dPAG 5-HT1A and 5-HT2C receptors interact each other in the modulation of OAA. 2018 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/17938-6 - Papel dos receptores serotoninérgicos da substância cinzenta periaquedutal sobre a antinocicepção de camundongos confinados aos braços abertos do labirinto em cruz elevado
• Beneficiário: Daniela Baptista de Souza
• Linha de fomento: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 10/06654-4 - Antinocicepção induzida pelos braços abertos do labirinto em cruz elevado em camundongos: avaliação da possível modulação pelos antidepressivos fluoxetina, sertralina e imipramina
• Beneficiário: Vinícius Pelarin Do Nascimento Costa
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/19472-4 - Participação do sistema histaminérgico em estruturas límbicas sobre a memória de esquiva inibitória em camundongos
• Beneficiário: Lucas Canto de Souza
• Linha de fomento: Bolsas no Brasil - Doutorado