Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Breathing responses produced by optogenetic stimulation of adrenergic C1 neurons are dependent on the connection with preBotzinger complex in rats

Texto completo
Autor(es):
Malheiros-Lima, Milene R. [1] ; Totola, Leonardo T. [1] ; Lana, Marlous V. G. [2] ; Strauss, Bryan E. [2] ; Takakura, Ana C. [3] ; Moreira, Thiago S. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Av Prof Lineu Prestes 1524, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Med, Canc Inst Sao Paulo, Ctr Translat Invest Oncol, LIM24, BR-01246000 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508000 Sao Paulo, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY; v. 470, n. 11, p. 1659-1672, NOV 2018.
Citações Web of Science: 0
Resumo

Optogenetic stimulation of the adrenergic C1 neurons produces cardiorespiratory activation, and selective depletion of these cells attenuates breathing responses induced by hypoxia. The preBotzinger complex (preBotC) is a group of neurons located in the intermediate aspect of the ventrolateral medulla, critical for respiratory rhythmogenesis, and is modulated by glutamate and catecholamines. Our hypothesis is that selective activation of C1 neurons leads to breathing responses by excitatory connections with the preBotC neurons. Anatomical connection between C1 cells and preBotC was evaluated using retrograde (Cholera Toxin b; preBotC) and anterograde (LVV-PRSx8-ChR2-eYFP; C1 region) tracers. LVV-PRSx8-ChR2-eYFP (viral vector that expresses channelrhodopsin-2 (ChR2) under the control of the catecholaminergic neuron-preferring promoter (PRSx8) was also injected into the C1 region of male Wistar rats for the functional experiments. Anatomical results demonstrated that preBotC neurons receive projections from C1 cells, and these projections express tyrosine hydroxylase and vesicular glutamate transporter 2. Functional connection between C1 cells and preBotC was evaluated by photostimulation of ChR2-transduced C1 neurons before and after unilateral injection of the ionotropic glutamate antagonist, kynurenic acid (kyn), or cocktail of adrenergic antagonists in the preBotC. Kyn injection into preBotC blocked the increase in Dia(EMG) frequency without changing the MAP increase elicited by photostimulation of C1 neurons, while the injection of adrenergic antagonists into the preBotC did not change Dia(EMG) frequency and MAP increase induced by photostimulation of C1 cells. Our results suggest that the increase in breathing produced by photostimulation of C1 neurons can be caused by a direct glutamatergic activation of preBotC neurons. (AU)

Processo FAPESP: 14/07698-6 - Envolvimento dos neurônios catecolaminérgicos do bulbo ventrolateral rostral no controle da atividade respiratória
Beneficiário:Milene Rodrigues Malheiros Lima
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 16/23281-3 - Regiões encefálicas responsáveis pela neuroplasticidade observada na resposta respiratória induzida por hipercapnia em modelo animal de doença de parkinson
Beneficiário:Ana Carolina Thomaz Takakura
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/23376-1 - Núcleo retrotrapezóide, quimiossensibilidade central e automaticidade respiratória
Beneficiário:Thiago dos Santos Moreira
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/22069-0 - Melhora nos mecanismos de regulação do fluxo sanguíneo encefálico em animais espontaneamente hipertensos: participação do exercício físico
Beneficiário:Thiago dos Santos Moreira
Linha de fomento: Auxílio à Pesquisa - Regular