Isolation and Characterization of Potentially Probiotic Bacterial Strains from Mice: Proof of Concept for Personalized Probiotics

Autor(es)

Celiberto, Larissa S. ^{[1, 2, 3]} ; Pinto, Roseli Aparecida ^[3] ; Rossi, Elizeu Antonio ^[3] ; Vallance, Bruce A. ^{[1, 2]} ; Cavallini, Daniela C. U. ^[3]

Afiliação do(s) autor(es)

^[1] BC Childrens Hosp, Div Gastroenterol, Dept Pediat, Vancouver, BC V5Z 4H4 - Canada

^[2] Univ British Columbia, Vancouver, BC V5Z 4H4 - Canada

^[3] Sao Paulo State Univ UNESP, Dept Food & Nutr, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRIENTS; v. 10, n. 11 NOV 2018.
• Citações Web of Science: 1

Resumo

Modulation of the gut microbiota through the use of probiotics has been widely used to treat or prevent several intestinal diseases. However, inconsistent results have compromised the efficacy of this approach, especially in severe conditions such as inflammatory bowel disease (IBD). The purpose of our study was to develop a personalized probiotic strategy and assess its efficacy in a murine model of intestinal inflammation. Commensal bacterial strains were isolated from the feces of healthy mice and then administered back to the host as a personalized treatment in dextran sodium sulfate (DSS)-induced colitis. Colonic tissues were collected for histological analysis and to investigate inflammatory markers such as Il-1 beta, Il-6, TGF-beta, and Il-10, and the enzyme myeloperoxidase as a neutrophil marker. The group that received the personalized probiotic showed reduced susceptibility to DSS-colitis as compared to a commercial probiotic. This protection was characterized by a lower disease activity index and reduced histopathological damage in the colon. Moreover, the personalized probiotic was more effective in modulating the host immune response, leading to decreased Il-1 beta and Il-6 and increased TGF-beta and Il-10 expression. In conclusion, our study suggests that personalized probiotics may possess an advantage over commercial probiotics in treating dysbiotic-related conditions, possibly because they are derived directly from the host's own microbiota. (AU)

• Processo FAPESP: 14/15129-1 - Isolamento e avaliação da eficácia bactérias láticas comensais e de uma bebida probiótica nos parâmetros inflamatórios e microbiológicos associados à colite ulcerativa
• Beneficiário: Daniela Cardoso Umbelino Cavallini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/15130-0 - Isolamento e avaliação da eficácia de bactérias láticas comensais nos parâmetros inflamatórios e microbiológicos associados à colite ulcerativa
• Beneficiário: Larissa Sbaglia Celiberto
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/21958-3 - Efeito do consumo de probiótico na microbiota fecal em camundongos induzidos por dextran sulfato de sódio
• Beneficiário: Larissa Sbaglia Celiberto
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado