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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation and Characterization of Potentially Probiotic Bacterial Strains from Mice: Proof of Concept for Personalized Probiotics

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Author(s):
Celiberto, Larissa S. [1, 2, 3] ; Pinto, Roseli Aparecida [3] ; Rossi, Elizeu Antonio [3] ; Vallance, Bruce A. [1, 2] ; Cavallini, Daniela C. U. [3]
Total Authors: 5
Affiliation:
[1] BC Childrens Hosp, Div Gastroenterol, Dept Pediat, Vancouver, BC V5Z 4H4 - Canada
[2] Univ British Columbia, Vancouver, BC V5Z 4H4 - Canada
[3] Sao Paulo State Univ UNESP, Dept Food & Nutr, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: NUTRIENTS; v. 10, n. 11 NOV 2018.
Web of Science Citations: 1
Abstract

Modulation of the gut microbiota through the use of probiotics has been widely used to treat or prevent several intestinal diseases. However, inconsistent results have compromised the efficacy of this approach, especially in severe conditions such as inflammatory bowel disease (IBD). The purpose of our study was to develop a personalized probiotic strategy and assess its efficacy in a murine model of intestinal inflammation. Commensal bacterial strains were isolated from the feces of healthy mice and then administered back to the host as a personalized treatment in dextran sodium sulfate (DSS)-induced colitis. Colonic tissues were collected for histological analysis and to investigate inflammatory markers such as Il-1 beta, Il-6, TGF-beta, and Il-10, and the enzyme myeloperoxidase as a neutrophil marker. The group that received the personalized probiotic showed reduced susceptibility to DSS-colitis as compared to a commercial probiotic. This protection was characterized by a lower disease activity index and reduced histopathological damage in the colon. Moreover, the personalized probiotic was more effective in modulating the host immune response, leading to decreased Il-1 beta and Il-6 and increased TGF-beta and Il-10 expression. In conclusion, our study suggests that personalized probiotics may possess an advantage over commercial probiotics in treating dysbiotic-related conditions, possibly because they are derived directly from the host's own microbiota. (AU)

FAPESP's process: 14/15129-1 - Isolation and evaluation of commensal lactic acid bacteria effectiveness and a probiotics beverage on inflammatory and microbiological parameters associated with ulcerative colitis
Grantee:Daniela Cardoso Umbelino Cavallini
Support Opportunities: Regular Research Grants
FAPESP's process: 14/15130-0 - Isolation and evaluation of commensal lactic acid bacteria effectiveness on inflammatory and microbiological parameters associated with ulcerative colitis
Grantee:Larissa Sbaglia Celiberto
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 15/21958-3 - Effect of probiotic consumption on the fecal microbiota in the dextran sodium sulphate colitis mouse model
Grantee:Larissa Sbaglia Celiberto
Support Opportunities: Scholarships abroad - Research Internship - Doctorate