Production of Omegas-6 and 9 from the Hydrolysis of Acai and Buriti Oils by Lipase Immobilized on a Hydrophobic Support

Autor(es):Mostrar menos -

Perez, Malena Martinez ^[1] ; Spiropulos Goncalves, Enrico Cerioni ^[1] ; Santos Salgado, Jose Carlos ^[2] ; Rocha, Mariana de Souza ^[1] ; de Almeida, Paula Zaghetto ^[1] ; Vici, Ana Claudia ^[3] ; Infante, Juliana da Conceicao ^[1] ; Manuel Guisan, Jose ^[4] ; Rocha-Martin, Javier ^[4] ; Costa Pessela, Benevides ^{[5, 6]} ; Teixeira de Moraes Polizeli, Maria de Lourdes ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bioquim & Imunol, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14049901 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14049901 Ribeirao Preto, SP - Brazil

^[4] CSIC, Inst Catalisis & Petroleoquim, Dept Biocatalisis, Campus UAM, Madrid 28049 - Spain

^[5] CIAL CSIC, Inst Ciencias Alimentac, Dept Biotecnol & Microbiol Alimentos, Campus UAM, Canto Blanco 28049 - Spain

^[6] Inst Super Politecn Tecnol & Ciencias ISPTEC, Dept Engn & Tecnol, Ave Luanda Sul, Rua Lateral Via S10, Talatona - Angola

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 23, n. 11 NOV 2018.
• Citações Web of Science: 2

Resumo

This paper describes a bioprocess to obtain omegas-6 and 9 from the hydrolysis of Acai (Euterpe oleracea Martius) and Buriti (Mauritia flexuosa) oils by lipases immobilized on octyl-sepharose. For this, oils and butters were initially selected as the carbon source which resulted in higher production of lipases in Beauveria bassiana and Fusarium oxysporum cultures. The carbon source that provided secretion of lipase by B. bassiana was Acai oil, and for F. oxysporum, Bacuri butter. Lipases obtained under these conditions were immobilized on octyl-sepharose, and both, the derivatives and the crude extracts were biochemically characterized. It was observed that the immobilization promoted an increase of stability in B. bassiana and F. oxysporum lipase activities at the given temperatures and pH. In addition, the immobilization promoted hyperactivation of B. bassiana and F. oxysporum lipase activities being 23.5 and 11.0 higher than free enzyme, respectively. The hydrolysis of Acai and Buriti oils by the derivatives was done in a biphasic (organic/aqueous) system, and the products were quantified in RP-HPLC. The results showed the potential of these immobilized lipases to obtain omegas-6 and 9 from Brazilian natural oils. This work may improve the enzymatic methodologies for obtaining foods and drugs enriched with fatty acids. (AU)

• Processo FAPESP: 13/50892-5 - Desenvolvimento de estratégias de imobilização e o uso de enzimas como biocatalizadores para aplicações industriais: produção de biodiesel e ingredientes funcionais
• Beneficiário: Maria de Lourdes Teixeira de Moraes Polizeli
• Modalidade de apoio: Auxílio à Pesquisa - Regular