Roux-en-Y Gastric Bypass Surgery Induces Distinct but Frequently Transient Effects on Acylcarnitine, Bile Acid and Phospholipid Levels

Autor(es)

Fiamoncini, Jarlei ^{[1, 2]} ; Barbosa, Carina Fernandes ^[3] ; Arnoni Junior, Jose Rubens ^[3] ; Araujo Junior, Jose Celestino ^[3] ; Taglieri, Cinthia ^[3] ; Szego, Tiago ^[4] ; Gelhaus, Barbara ^[2] ; de Souza, Heraldo Possolo ^[5] ; Daniel, Hannelore ^[2] ; de Lima, Thais Martins ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Food Sci & Expt Nutr, BR-05508060 Sao Paulo - Brazil

^[2] Tech Univ Munich, Nutr & Food Sci, D-85354 Freising Weihenstephan - Germany

^[3] Clin IMEC, BR-04260020 Sao Paulo - Brazil

^[4] Inst CIGO, BR-05508060 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Lab Emergencias Clin LIM 51, BR-05508060 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: METABOLITES; v. 8, n. 4 DEC 2018.
• Citações Web of Science: 0

Resumo

Roux-en-Y gastric bypass (RYGB) is an effective method to achieve sustained weight loss, but the mechanisms responsible for RYGB effects have not yet been fully characterized. In this study, we profiled the concentrations of 143 lipid metabolites in dry blood spots (DBS) of RYGB patients. DBS from obese patients (BMI range 35-44 kg/m(2)) were collected 7 days before, 15 and 90 days after the surgery. LC-MS/MS was used to quantify acylcarnitines, phosphatidylcholines, sphingomyelins and bile acids. RYGB caused a rapid increase in acylcarnitine levels that proved to be only transient, contrasting with the sustained decrease in phosphatidylcholines and increase of sphingomyelins and bile acids. A PLS-DA analysis revealed a 3-component model (R-2 = 0.9, Q(2) = 0.74) with key metabolites responsible for the overall metabolite differences. These included the BCAA-derived acylcarnitines and sphingomyelins with 16 and 18 carbons. We found important correlations between the levels of BCAA-derived acylcarnitines and specific sphingomyelins with plasma cholesterol and triacylglycerol concentrations. Along with the marked weight loss and clinical improvements, RYGB induced specific alterations in plasma acylcarnitines, bile acid and phospholipid levels. This calls for more studies on RYGB effects aiming to elucidate the metabolic adaptations that follow this procedure. (AU)

• Processo FAPESP: 14/05260-3 - Acil-carnitinas como marcadores do metabolismo intermediário em indivíduos com obesidade mórbida submetidos a uma intervenção clínica para perda de peso
• Beneficiário: Thais Martins de Lima Salgado
• Modalidade de apoio: Auxílio à Pesquisa - Regular