Therapeutic effects of vaccine derived from amastigote surface protein-2 (ASP-2) against Chagas disease in mouse liver

Autor(es)

Pidone Ribeiro, Flavia Andressa ^[1] ; Pontes, Camila ^[2] ; Gazzinelli, Ricardo T. ^[3] ; Romero, Oscar-Bruna ^[4] ; Lazzarin, Mariana Cruz ^[1] ; dos Santos, Jose Fontes ^[1] ; de Oliveira, Flavia ^[1] ; Pisani, Luciana Pellegrini ^[1] ; Carvalho de Vasconcelos, Jose Ronnie ^[1] ; Ribeiro, Daniel Araki ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Biociencias, Campus Baixada Santista, Santos, SP - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med, CTCMol, Sao Paulo, SP - Brazil

^[3] Univ Massachusetts, Dept Imunol, Amherst, MA 01003 - USA

^[4] Univ Fed Minas Gerais, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CYTOKINE; v. 113, p. 285-290, JAN 2019.
• Citações Web of Science: 1

Resumo

This study investigated the efficacy of the vaccine in liver of mice infected with the Trypanosoma cruzi (T. cruzi) and immunized with AdASP-2. For this purpose, histopathological analysis and gene expression of COX-2, TNFalpha, TNFR, iNOS, cytochrome C, caspase-3, TLR4, IL-6 and IL10 were evaluated. The following groups were used in this study: Group 1- Control Group (CTRL) animals received Adj3Gal vehicle; Group 2- Infected Group (TC) animals were infected with T. cruzi; Group 3- Immunized Group (AdASP-2): animals were immunized by AdASP-2 vaccine; Group 4- Immunized and Infected Group (AdASP-2 + TC) animals were infected with T. cruzi and immunized by AdSP-2 vaccine. A significant decrease of amastigote nests was noticed in the group of animals that were immunized with AdASP-2 and infected on the same day. COX-2 and TNF-alpha gene expressions increased in TC group, whereas TNF-alpha decreased in the TC + AdASP-2 group. TNFR expression was high in AdASP-2 + TC group. iNOS expression was high for all experimental groups whereas cytochrome C decreased for all experimental groups. Caspase 3 increased in TC and TC + AdASP-2 groups. The gene expression of TLR4 and IL-10 showed an increase in AdASP-2 + TC group. Finally, hepatic fibrosis was noticed to TC and AdASP-2 + TC groups. Taken together, our results demonstrated that vaccination with AdASP-2 was effective against the acute phase of experimental Chagas disease as a result of a more powerful and rapid immune response closely related to expression of some inflammatory genes, such as iNOS, TNF-alpha, TLR 4, and IL-10. (AU)

• Processo FAPESP: 12/22514-3 - Estudo da migração de células T específicas geradas pela vacinação ou infecção pelo Trypanosoma cruzi
• Beneficiário: Jose Ronnie Carvalho de Vasconcelos
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores