| Full text | |
| Author(s): |
Pidone Ribeiro, Flavia Andressa
[1]
;
Pontes, Camila
[2]
;
Gazzinelli, Ricardo T.
[3]
;
Romero, Oscar-Bruna
[4]
;
Lazzarin, Mariana Cruz
[1]
;
dos Santos, Jose Fontes
[1]
;
de Oliveira, Flavia
[1]
;
Pisani, Luciana Pellegrini
[1]
;
Carvalho de Vasconcelos, Jose Ronnie
[1]
;
Ribeiro, Daniel Araki
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Biociencias, Campus Baixada Santista, Santos, SP - Brazil
[2] Univ Fed Sao Paulo, Escola Paulista Med, CTCMol, Sao Paulo, SP - Brazil
[3] Univ Massachusetts, Dept Imunol, Amherst, MA 01003 - USA
[4] Univ Fed Minas Gerais, Belo Horizonte, MG - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | CYTOKINE; v. 113, p. 285-290, JAN 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
This study investigated the efficacy of the vaccine in liver of mice infected with the Trypanosoma cruzi (T. cruzi) and immunized with AdASP-2. For this purpose, histopathological analysis and gene expression of COX-2, TNFalpha, TNFR, iNOS, cytochrome C, caspase-3, TLR4, IL-6 and IL10 were evaluated. The following groups were used in this study: Group 1- Control Group (CTRL) animals received Adj3Gal vehicle; Group 2- Infected Group (TC) animals were infected with T. cruzi; Group 3- Immunized Group (AdASP-2): animals were immunized by AdASP-2 vaccine; Group 4- Immunized and Infected Group (AdASP-2 + TC) animals were infected with T. cruzi and immunized by AdSP-2 vaccine. A significant decrease of amastigote nests was noticed in the group of animals that were immunized with AdASP-2 and infected on the same day. COX-2 and TNF-alpha gene expressions increased in TC group, whereas TNF-alpha decreased in the TC + AdASP-2 group. TNFR expression was high in AdASP-2 + TC group. iNOS expression was high for all experimental groups whereas cytochrome C decreased for all experimental groups. Caspase 3 increased in TC and TC + AdASP-2 groups. The gene expression of TLR4 and IL-10 showed an increase in AdASP-2 + TC group. Finally, hepatic fibrosis was noticed to TC and AdASP-2 + TC groups. Taken together, our results demonstrated that vaccination with AdASP-2 was effective against the acute phase of experimental Chagas disease as a result of a more powerful and rapid immune response closely related to expression of some inflammatory genes, such as iNOS, TNF-alpha, TLR 4, and IL-10. (AU) | |
| FAPESP's process: | 12/22514-3 - Migration study of specific T cells generated by vaccination or Trypanosoma cruzi infection |
| Grantee: | Jose Ronnie Carvalho de Vasconcelos |
| Support Opportunities: | Research Grants - Young Investigators Grants |