Nighttime light exposure enhances Rev-erb alpha-targeting microRNAs and contributes to hepatic steatosis

Autor(es)

Borck, Patricia C. ^{[1, 2]} ; Batista, Thiago M. ^{[1, 2]} ; Vettorazzi, Jean F. ^{[1, 2]} ; Soares, Gabriela M. ^{[1, 2]} ; Lubaczeuski, Camila ^{[1, 2]} ; Guan, Dongyin ^{[3, 4]} ; Boschero, Antonio C. ^{[1, 2]} ; Vieira, Elaine ^[5] ; Lazar, Mitchell A. ^{[3, 4]} ; Carneiro, Everardo M. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, Obes & Comorbid Res Ctr, Campinas, SP - Brazil

^[3] Univ Penn, Perelman Sch Med, Dept Med, Div Endocrinol Diabet & Metab, Philadelphia, PA 19104 - USA

^[4] Univ Penn, Perelman Sch Med, Inst Diabet, Philadelphia, PA 19104 - USA

^[5] Univ Catolica Brasilia, Postgrad Program Phys Educ, Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: METABOLISM-CLINICAL AND EXPERIMENTAL; v. 85, p. 250-258, AUG 2018.
• Citações Web of Science: 1

Resumo

Objective: The exposure to artificial light at night (ALAN) disrupts the biological rhythms and has been associated with the development of metabolic syndrome. MicroRNAs (miRNAs) display a critical role in fine-tuning the circadian system and energy metabolism. In this study, we aimed to assess whether altered miRNAs expression in the liver underlies metabolic disorders caused by disrupted biological rhythms. Results: We found that C3H/HePas mice exposed to ALAN developed obesity, and hepatic steatosis, which was paralleled by decreased expression of Rev-erb alpha and up-regulation of its lipogenic targets ACL and FAS in liver. Furthermore, the expression of Rev-erb alpha-targeting miRNAs, miR-140-5p, 185-5p, 326-5p and 328-5p were increased in this group. Consistently, overexpression of these miRNAs in primary hepatocytes reduced Rev-erb alpha expression at the mRNA and protein levels. Importantly, overexpression of Rev-erb alpha-targeting miRNAs increased mRNA levels of Acly and Fasn. Conclusion: Thus, altered miRNAs profile is an important mechanism underlying the disruption of the peripheral clock caused by exposure to ALAN, which could lead to hepatic steatosis. (C) 2018 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 13/26740-0 - Análise molecular e funcional do mecanismo de secreção e ação da insulina em camundongos desnutridos submetidos a exposição contínua a luz
• Beneficiário: Patricia Cristine Borck
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/01717-9 - Investigação dos efeitos insulinotrópicos, insulinomiméticos e endoteliais da taurina em células/tecidos submetidos à restrição in vitro de aminoácidos: uma abordagem integrada e multifocal
• Beneficiário: Everardo Magalhães Carneiro
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/12611-0 - Mecanismos moleculares envolvidos na disfunção e morte de células beta pancreáticas no Diabetes mellitus: estratégias para a inibição desses processos e para a recuperação da massa insular
• Beneficiário: Antonio Carlos Boschiero
• Modalidade de apoio: Auxílio à Pesquisa - Temático