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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nighttime light exposure enhances Rev-erb alpha-targeting microRNAs and contributes to hepatic steatosis

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Borck, Patricia C. [1, 2] ; Batista, Thiago M. [1, 2] ; Vettorazzi, Jean F. [1, 2] ; Soares, Gabriela M. [1, 2] ; Lubaczeuski, Camila [1, 2] ; Guan, Dongyin [3, 4] ; Boschero, Antonio C. [1, 2] ; Vieira, Elaine [5] ; Lazar, Mitchell A. [3, 4] ; Carneiro, Everardo M. [1, 2]
Total Authors: 10
[1] Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Inst Biol, Obes & Comorbid Res Ctr, Campinas, SP - Brazil
[3] Univ Penn, Perelman Sch Med, Dept Med, Div Endocrinol Diabet & Metab, Philadelphia, PA 19104 - USA
[4] Univ Penn, Perelman Sch Med, Inst Diabet, Philadelphia, PA 19104 - USA
[5] Univ Catolica Brasilia, Postgrad Program Phys Educ, Brasilia, DF - Brazil
Total Affiliations: 5
Document type: Journal article
Source: METABOLISM-CLINICAL AND EXPERIMENTAL; v. 85, p. 250-258, AUG 2018.
Web of Science Citations: 1

Objective: The exposure to artificial light at night (ALAN) disrupts the biological rhythms and has been associated with the development of metabolic syndrome. MicroRNAs (miRNAs) display a critical role in fine-tuning the circadian system and energy metabolism. In this study, we aimed to assess whether altered miRNAs expression in the liver underlies metabolic disorders caused by disrupted biological rhythms. Results: We found that C3H/HePas mice exposed to ALAN developed obesity, and hepatic steatosis, which was paralleled by decreased expression of Rev-erb alpha and up-regulation of its lipogenic targets ACL and FAS in liver. Furthermore, the expression of Rev-erb alpha-targeting miRNAs, miR-140-5p, 185-5p, 326-5p and 328-5p were increased in this group. Consistently, overexpression of these miRNAs in primary hepatocytes reduced Rev-erb alpha expression at the mRNA and protein levels. Importantly, overexpression of Rev-erb alpha-targeting miRNAs increased mRNA levels of Acly and Fasn. Conclusion: Thus, altered miRNAs profile is an important mechanism underlying the disruption of the peripheral clock caused by exposure to ALAN, which could lead to hepatic steatosis. (C) 2018 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 14/01717-9 - Investigation of the insulinotropic, insulinomimetic and endothelial actions of taurine in cells/tissues submitted to an in vitro amino acid restriction: an integrated and multifocal approach
Grantee:Everardo Magalhães Carneiro
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/12611-0 - Molecular mechanisms involved in pancreatic beta cell disfunction and dead in diabetes mellitus: strategies for the inhibition of these processes and restoration of the insular mass
Grantee:Antonio Carlos Boschiero
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/26740-0 - Molecular and functional analysis of the mechanism of secretion and insulin action in malnourished mice subjected to continuous exposure to light
Grantee:Patricia Cristine Borck
Support type: Scholarships in Brazil - Doctorate