Molecular and functional analysis of the mechanism of secretion and insulin action in malnourished mice subjected to continuous exposure to light

• Grant number: 13/26740-0
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): July 01, 2014
• Effective date (End): December 31, 2017
• Field of knowledge: Biological Sciences - Physiology
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Everardo Magalhães Carneiro
• Grantee: Patricia Cristine Borck
• Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 16/04314-8 - Control of Rev-erb-alpha expression after disruption of circadian rhythm, BE.EP.DR

Abstract

Currently, increasing prevalence of obesity and type 2 diabetes (DM2) correlates with disruption of circadian rhythm caused by higher exposure of light at night (LAN), due to restriction of sleep, shift work, jet lag and artificial light in houses, streets and work and leisure environments. Moreover, exposure LAN when diet associated with altered nutrient supply, such as high fat (HFD), triggers more marked changes in lipid and glucose homeostasis accelerating the development of obesityIn this context, the microRNA (miRNA) represents an emerging class of regulators of energy homeostasis, which can to be modulated by light and circadian rhythm. The microRNAs are small no-coding RNA, which act in the control of gene expression, pos-transcriptional level, leading to inhibition or reduction of protein synthesis. In patophysiological states, such as malnutrition, obesity and DM2 the mechanisms of synthesis and maturation of microRNA are changed, fact that favors to progression these diseases.Over years, our research group has characterized the pancreatic function in protein-malnourished mice. These animals show lower glucose stimulated insulin secretion and other insulinotropic agents, as well as higher glucose tolerance and insulin peripheral sensibility. Moreover, malnourished mice exhibit alteration in clock genes expression and pattern circadian of insulin secretion. We believe, these circadian changes predispose, protein-malnutrition mice, to be more susceptible to metabolic changes induced to exposure light continues. Thus we aim to assess the effects of exposure the LAN on function ²-pancreatic and peripheral tissues in the control of glucose homeostasis in malnourished mice. Furthermore, we propose to determine a possible role of miRNA as mediators of changes in insulin secretion in this experimental model. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORCK, PATRICIA C.; BATISTA, THIAGO M.; VETTORAZZI, JEAN F.; SOARES, GABRIELA M.; LUBACZEUSKI, CAMILA; GUAN, DONGYIN; BOSCHERO, ANTONIO C.; VIEIRA, ELAINE; LAZAR, MITCHELL A.; CARNEIRO, EVERARDO M. Nighttime light exposure enhances Rev-erb alpha-targeting microRNAs and contributes to hepatic steatosis. METABOLISM-CLINICAL AND EXPERIMENTAL, v. 85, p. 250-258, AUG 2018. Web of Science Citations: 1.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BORCK, Patricia Cristine. Functional and molecular analysis of energy metabolism from mice exposed to artificial light at night and fed or not to low-protein diet. 2018. Doctoral Thesis - Universidade Estadual de Campinas, Instituto de Biologia.