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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Cytotoxic Activity of Fatty Acids From Antarctic Macroalgae on the Growth of Human Breast Cancer Cells

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Autor(es):
Pacheco, Bruna Silveira [1, 2] ; Ziemann dos Santos, Marco Aurelio [1] ; Schultze, Eduarda [2] ; Martins, Rosiane Mastelari [1] ; Lund, Rafael Guerra [1] ; Seixas, Fabiana Kommling [2] ; Colepicolo, Pio [3] ; Collares, Tiago [1, 2] ; Paula, Favero Reisdorfer [4] ; Pereira De Pereira, Claudio Martin [1, 2]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Fed Pelotas, Postgrad Program Biochem & Bioprospect, Lipid & Bioorgan Lab, Bioforens Res Grp, Pelotas - Brazil
[2] Univ Fed Pelotas, Res Grp Cellular & Mol Oncol, Postgrad Program Biotechnol, Pelotas - Brazil
[3] Univ Sao Paulo, Chem Inst, Sao Paulo - Brazil
[4] Fed Univ Pampa, Pharmaceut Sci Postgrad Program, Lab Res & Drugs Dev, Bage - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY; v. 6, DEC 3 2018.
Citações Web of Science: 1
Resumo

Macroalgae are a natural source of clinically relevant molecules such as polyunsaturated and monounsaturated fatty acids. The Antarctic environment, due to its cold climate, leads to high production of these bioactive molecules. Adenocystis utricularis, Curdiea racovitzae, and Georgiella confluens from three distinct islands in the Antarctic Peninsula were collected and analyzed for their fatty acid content by gas chromatography flame ionization detection. Results revealed that the algal extracts consisted of 22 fatty acids, of which 9 were saturated, 4 were monounsaturated, and 9 were polyunsaturated (PUFA). In addition, fucosterol was identified within the lipidic extracts. The cytotoxic activity of these fatty acids was evaluated in human breast cancer cell lines MCF-7 and MDA-MB-231. The most notable result was the effect of PUFA on the growth inhibition of cancer cells ranging from 61.04 to 69.78% in comparison to control cells. Significant cytotoxic activity of fatty acids from A. utricularis was observed at 48 h, resulting in an inhibition of growth of more than 50% for breast cancer cells at a concentration of 100 mu g/mL. A cell viability assay showed that the fatty acids from A. utricularis significantly reduced cell viability (68.7% in MCF-7 and 89% in MDA-MB-231 after 72 h of exposure). At the same time, DAPI staining demonstrated chromatin condensation, and apoptotic bodies formed in cells that were cultured with fatty acids from A. utricularis. These data indicate that fatty acids from Antarctic macroalgae have the potential to reduce the proliferation of and induce apoptosis in breast cancer cells. (AU)

Processo FAPESP: 10/50193-1 - Estudos de bioprospecção de macroalgas marinhas, uso da biomassa algal como fonte de novos fármacos e bioativos economicamente viáveis e sua aplicação na remediação de áreas impactadas. (Biodiversidade marinha)
Beneficiário:Pio Colepicolo Neto
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático