Using natural deep eutectic solvents for the extraction of metabolites in Byrsonima intermedia leaves

Autor(es)

Fraige, Karina ^[1] ; Arrua, R. Dario ^[2] ; Sutton, Adam T. ^[2] ; Funari, Cristiano Soleo ^[3] ; Cavalheiro, Alberto Jose ^[1] ; Hilder, Emily F. ^[2] ; Bolzani, Vanderlan da Silva ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Sao Paulo State Univ, Inst Chem, BR-14800060 Araraquara, SP - Brazil

^[2] Univ South Australia UniSA, Future Ind Inst, Adelaide, SA - Australia

^[3] UNESP Sao Paulo State Univ, Fac Agr Sci, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SEPARATION SCIENCE; v. 42, n. 2, p. 591-597, JAN 2019.
• Citações Web of Science: 2

Resumo

Natural deep eutectic solvents have been used as an alternative to organic solvents for the extraction of plants metabolites, allowing for the extraction of compounds of different polarities, while being inexpensive, non-toxic, and easy to prepare. This work presents the comparison of the chromatographic profiles by high-performance liquid chromatography with diode-array detection obtained from Byrsonima intermedia (Malpighiaceae) using five choline chloride-based natural deep eutectic solvents, in addition to the most used traditional extraction solvents, methanol/water 7: 3 and ethanol/water 7: 3 v/v. A reference extract was used to tentatively identify compounds by high-performance liquid chromatography with tandem mass spectrometry. The water content appeared to be important for the extraction efficiency and the mixture choline chloride/glycerol was shown to be the best candidate for efficiently extracting this matrix when compared with the traditional extraction media in addition to being far greener as shown by the environmental analysis tool. Seven phenolic compounds (digalloyl quinic acid, proanthocyanidin dimer, galloylproanthocyanidin dimer, quercetin-O-hexoside, galloyl quercetin hexoside, quercetin-O-pentoside, and galloyl quercetin pentoside) were tentatively identified in all extracts. Moreover, the influence of these solvents on the antioxidant activity of the extracts was studied and the results for choline chloride/glycerol extracts were very similar to that of the traditional extraction solvents. (AU)

• Processo FAPESP: 13/15086-8 - Aplicações da metabolômica e proteômica na busca por novas substâncias biologicamente ativas em espécies da família Malpighiaceae
• Beneficiário: Karina Fraige Baraco
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos
• Beneficiário: Glaucius Oliva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 16/50009-2 - Greening sample preparation of complex matrices by using natural deep eutectic solvents (NADES) together with porous polymeric adsorbents for subsequent analyses by liquid chromatography (LC) and nuclear magnetic resonance (NMR)
• Beneficiário: Vanderlan da Silva Bolzani
• Modalidade de apoio: Auxílio à Pesquisa - Regular