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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Using natural deep eutectic solvents for the extraction of metabolites in Byrsonima intermedia leaves

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Author(s):
Fraige, Karina [1] ; Arrua, R. Dario [2] ; Sutton, Adam T. [2] ; Funari, Cristiano Soleo [3] ; Cavalheiro, Alberto Jose [1] ; Hilder, Emily F. [2] ; Bolzani, Vanderlan da Silva [1]
Total Authors: 7
Affiliation:
[1] UNESP Sao Paulo State Univ, Inst Chem, BR-14800060 Araraquara, SP - Brazil
[2] Univ South Australia UniSA, Future Ind Inst, Adelaide, SA - Australia
[3] UNESP Sao Paulo State Univ, Fac Agr Sci, Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF SEPARATION SCIENCE; v. 42, n. 2, p. 591-597, JAN 2019.
Web of Science Citations: 2
Abstract

Natural deep eutectic solvents have been used as an alternative to organic solvents for the extraction of plants metabolites, allowing for the extraction of compounds of different polarities, while being inexpensive, non-toxic, and easy to prepare. This work presents the comparison of the chromatographic profiles by high-performance liquid chromatography with diode-array detection obtained from Byrsonima intermedia (Malpighiaceae) using five choline chloride-based natural deep eutectic solvents, in addition to the most used traditional extraction solvents, methanol/water 7: 3 and ethanol/water 7: 3 v/v. A reference extract was used to tentatively identify compounds by high-performance liquid chromatography with tandem mass spectrometry. The water content appeared to be important for the extraction efficiency and the mixture choline chloride/glycerol was shown to be the best candidate for efficiently extracting this matrix when compared with the traditional extraction media in addition to being far greener as shown by the environmental analysis tool. Seven phenolic compounds (digalloyl quinic acid, proanthocyanidin dimer, galloylproanthocyanidin dimer, quercetin-O-hexoside, galloyl quercetin hexoside, quercetin-O-pentoside, and galloyl quercetin pentoside) were tentatively identified in all extracts. Moreover, the influence of these solvents on the antioxidant activity of the extracts was studied and the results for choline chloride/glycerol extracts were very similar to that of the traditional extraction solvents. (AU)

FAPESP's process: 13/15086-8 - Application of metabolomics and proteomics in searching for new biologically active compounds in Malpighiaceae plant species
Grantee:Karina Fraige Baraco
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
Grantee:Glaucius Oliva
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 16/50009-2 - Greening sample preparation of complex matrices by using natural deep eutectic solvents (NADES) together with porous polymeric adsorbents for subsequent analyses by liquid chromatography (LC) and nuclear magnetic resonance (NMR)
Grantee:Vanderlan da Silva Bolzani
Support Opportunities: Regular Research Grants