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Application of metabolomics and proteomics in searching for new biologically active compounds in Malpighiaceae plant species

Grant number: 13/15086-8
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2014
Effective date (End): December 31, 2016
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Vanderlan da Silva Bolzani
Grantee:Karina Fraige Baraco
Home Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated research grant:13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery, AP.CEPID

Abstract

The use of natural products date from antiquity, and they are known on the treatment of various diseases, being considered one of the main sources of drugs due to present a wide range of chemical compounds and biological functions. Worldwide, particularly in Brazil, which holds a huge biodiversity, only a small fraction of these organisms is explored. Recently, with the improvement of analytical techniques as nuclear magnetic resonance (NMR) and mass spectrometry (MS), and metabolomics approaches and dereplication, which allow to distinguish new compounds from that already described, the number of studied organisms has increased, being the plants a main focus with good results. Malpighiaceae is a tropical family of plants occurring in the most part of Brazil, and known for its antimicrobial activity. Preliminary tests on Núcleo de Bioensaios, Biossíntese e Ecofisiologia de Produtos Naturais (NuBBE) indicated anticholinesterasic activity of some species from this family, making interesting the searching for the compounds responsible for the activity that is related with Alzheimer's disease. Therefore, the objective of this work is to study the metabolomic profile of these plants by MS and NMR, applying dereplication approaches for the isolation of novel compounds. Moreover, we intend to evaluate the proteomic profile and activity tests with the protein extracts, since proteins can help to understand the production of the interest metabolites. There are few studies about proteomics of the Brazilian medicinal plants, and this subject can be valuable taking in account that proteins have several important biological properties that may also have interesting pharmacological properties.

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FUNARI, CRISTIANO SOLEO; SUTTON, ADAM T.; CARNEIRO, RENATO LAJARIM; FRAIGE, KARINA; CAVALHEIRO, ALBERTO JOSE; BOLZANI, VANDERLAN DA SILVA; HILDER, EMILY F.; DARIO ARRUA, R. Natural deep eutectic solvents and aqueous solutions as an alternative extraction media for propolis. Food Research International, v. 125, NOV 2019. Web of Science Citations: 0.
FRAIGE, KARINA; ARRUA, R. DARIO; SUTTON, ADAM T.; FUNARI, CRISTIANO SOLEO; CAVALHEIRO, ALBERTO JOSE; HILDER, EMILY F.; BOLZANI, VANDERLAN DA SILVA. Using natural deep eutectic solvents for the extraction of metabolites in Byrsonima intermedia leaves. JOURNAL OF SEPARATION SCIENCE, v. 42, n. 2, p. 591-597, JAN 2019. Web of Science Citations: 2.
SUTTON, ADAM T.; FRAIGE, KARINA; LEME, GABRIEL MAZZI; BOLZANI, VANDERLAN DA SILVA; HILDER, EMILY F.; CAVALHEIRO, ALBERTO J.; ARRUA, R. DARIO; FUNARI, CRISTIANO SOLEO. Natural deep eutectic solvents as the major mobile phase components in high-performance liquid chromatography-searching for alternatives to organic solvents. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 410, n. 16, p. 3705-3713, JUN 2018. Web of Science Citations: 8.
FRAIGE, KARINA; DAMETTO, ALESSANDRA CRISTINA; ZERAIK, MARIA LUIZA; DE FREITAS, LARISSA; SARAIVA, AMANDA CORREIA; MEDEIROS, ALEXANDRA IVO; CASTRO-GAMBOA, IAN; CAVALHEIRO, ALBERTO JOSE; SILVA, DULCE HELENA S.; LOPES, NORBERTO PEPORINE; BOLZANI, VANDERLAN S. Dereplication by HPLC-DAD-ESI-MS/MS and Screening for Biological Activities of Byrsonima Species (Malpighiaceae). Phytochemical Analysis, v. 29, n. 2, p. 196-204, MAR-APR 2018. Web of Science Citations: 12.

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