Therapeutical effects of vaccine from Trypanosoma cruzi amastigote surface protein 2 by simultaneous inoculation with live parasites

Autor(es)

Pidone Ribeiro, Flavia Andressa ^[1] ; Pontes, Camila ^[2] ; Machado, Alexandre De M. V. ^[3] ; Bruna-Romero, Oscar ^[4] ; Quintana, Hananiah T. ^[1] ; De Oliveira, Flavia ^[1] ; Carvalho De Vasconcelos, Jose Ronnie ^[1] ; Ribeiro, Daniel Araki ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Biociencias, Campus Baixada Santista, Santos - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med, Ctr Terapia Celular & Mol CTCMol, Sao Paulo, SP - Brazil

^[3] Fiocruz Minas Gerais, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG - Brazil

^[4] Univ Fed Minas Gerais, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Biochemistry; v. 120, n. 3, p. 3373-3383, MAR 2019.
• Citações Web of Science: 0

Resumo

The aim of this study was to evaluate the efficacy of vaccine using replication-deficient human recombinant Type 5 replication-defective adenoviruses (AdHu5) carrying sequences of the amastigote surface protein 2 (ASP2) (AdASP2) in mice infected with the Trypanosoma cruzi (T cruzi) Y strain. A total of 16 A/Sn mice female were distributed into four groups, as follows (n = 4 per group): Group 1 - Control Group (CTRL); Group 2 - Infected Group (TC): animals were infected by subcutaneous route with 150 bloodstream trypomastigotes of T cruzi Y strain; Group 3 - Immunized Group (AdASP-2): animals were immunized by intramuscular injection (im) route with 50 mu L of AdSP-2 (2 x 10(8) plaque forming units {[}pfu]/cam) at day 0; Group 4-Immunized and Infected Group (AdASP-2+TC): animals were immunized by im route with 50 mu L of ASP-2 (2 x 10(8) pfu/cam) and infected by T cruzi at the same day (day 0). It was observed a significant decrease of nests in the group that was immunized with AdASP-2 and infected on the same day. Tumor necrosis factor alpha (TNF-alpha) and inducible nitric oxide synthase (iNOS) gene expressions showed a significant increase in the AdASP-2+TC group when compared to TC group, but it was noted that Cyclooxygenase-2 (Cox-2) was increased in TC group when compared to AdASP-2+TC group. Increase of matrix metalloproteinases-2 (MMP-2) and decrease of MMP-9 immunoexpression in the AdASP-2+TC group was noticed as well. Oxidative DNA damage was present in myocardium for AdASP-2+TC group as a result of 8-hydroxydeoxyguanosine immunoexpression. Taken together, our results highlighted an increased oxidative stress, MMP-2 activity and inflammatory host response promoted by AdASP-2 against T cruzi infection. (AU)

• Processo FAPESP: 12/22514-3 - Estudo da migração de células T específicas geradas pela vacinação ou infecção pelo Trypanosoma cruzi
• Beneficiário: Jose Ronnie Carvalho de Vasconcelos
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores