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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Therapeutical effects of vaccine from Trypanosoma cruzi amastigote surface protein 2 by simultaneous inoculation with live parasites

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Author(s):
Pidone Ribeiro, Flavia Andressa [1] ; Pontes, Camila [2] ; Machado, Alexandre De M. V. [3] ; Bruna-Romero, Oscar [4] ; Quintana, Hananiah T. [1] ; De Oliveira, Flavia [1] ; Carvalho De Vasconcelos, Jose Ronnie [1] ; Ribeiro, Daniel Araki [1]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Paulo, Dept Biociencias, Campus Baixada Santista, Santos - Brazil
[2] Univ Fed Sao Paulo, Escola Paulista Med, Ctr Terapia Celular & Mol CTCMol, Sao Paulo, SP - Brazil
[3] Fiocruz Minas Gerais, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG - Brazil
[4] Univ Fed Minas Gerais, Belo Horizonte, MG - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Cellular Biochemistry; v. 120, n. 3, p. 3373-3383, MAR 2019.
Web of Science Citations: 0
Abstract

The aim of this study was to evaluate the efficacy of vaccine using replication-deficient human recombinant Type 5 replication-defective adenoviruses (AdHu5) carrying sequences of the amastigote surface protein 2 (ASP2) (AdASP2) in mice infected with the Trypanosoma cruzi (T cruzi) Y strain. A total of 16 A/Sn mice female were distributed into four groups, as follows (n = 4 per group): Group 1 - Control Group (CTRL); Group 2 - Infected Group (TC): animals were infected by subcutaneous route with 150 bloodstream trypomastigotes of T cruzi Y strain; Group 3 - Immunized Group (AdASP-2): animals were immunized by intramuscular injection (im) route with 50 mu L of AdSP-2 (2 x 10(8) plaque forming units {[}pfu]/cam) at day 0; Group 4-Immunized and Infected Group (AdASP-2+TC): animals were immunized by im route with 50 mu L of ASP-2 (2 x 10(8) pfu/cam) and infected by T cruzi at the same day (day 0). It was observed a significant decrease of nests in the group that was immunized with AdASP-2 and infected on the same day. Tumor necrosis factor alpha (TNF-alpha) and inducible nitric oxide synthase (iNOS) gene expressions showed a significant increase in the AdASP-2+TC group when compared to TC group, but it was noted that Cyclooxygenase-2 (Cox-2) was increased in TC group when compared to AdASP-2+TC group. Increase of matrix metalloproteinases-2 (MMP-2) and decrease of MMP-9 immunoexpression in the AdASP-2+TC group was noticed as well. Oxidative DNA damage was present in myocardium for AdASP-2+TC group as a result of 8-hydroxydeoxyguanosine immunoexpression. Taken together, our results highlighted an increased oxidative stress, MMP-2 activity and inflammatory host response promoted by AdASP-2 against T cruzi infection. (AU)

FAPESP's process: 12/22514-3 - Migration study of specific T cells generated by vaccination or Trypanosoma cruzi infection
Grantee:Jose Ronnie Carvalho de Vasconcelos
Support Opportunities: Research Grants - Young Investigators Grants