Effect of EGF on expression and localization of maturation-promoting factor, mitogen-activated protein kinase, p34(cdc2) and cyclin B during different culture periods on in vitro maturation of canine oocytes

Autor(es)

Pereira, Leda Maria C. ^[1] ; Bersano, Paulo Ricardo O. ^[2] ; Rocha, Danilo D. ^[3] ; Lopes, Maria Denise ^[1]

Afiliação do(s) autor(es)

^[1] FMVZ UNESP, Dept Anim Reprod & Vet Radiol, Botucatu, SP - Brazil

^[2] Univ Estadual Ceara, Fac Vet FAVET, Fortaleza, Ceara - Brazil

^[3] Univ Fed Ceara, Dept Physiol & Pharmacol, Fortaleza, Ceara - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION IN DOMESTIC ANIMALS; v. 54, n. 2, p. 325-341, FEB 2019.
• Citações Web of Science: 2

Resumo

Contents This study aimed to investigate the localization of MPF, MAPK, p34(cdc2) and cyclin B1 proteins, before and after treatment with EGF during different moments of oocyte maturation. The ovaries obtained from 350 domestic dogs were aseptically isolated, immersed in physiological solution and transported at 4 degrees C. In the laboratory, the ovaries were sectioned for the release of cumulus-oocyte complexes. Cumulus-oocyte complexes were selected and divided into treatment groups with and without EGF and cultured for 24, 48 and 72hr. Immunofluorescence was used for the detection and the localization of MAPK, MPF, p34(cdc2) and cyclin B1 proteins. We observed that the expression and localization of MPF, MAPK, p34(cdc2) and cyclin B1 proteins are associated with meiosis resumption and cell cycle progression, and that EGF influences cell signalling pathways by promoting alterations in the localization of these proteins, improving the acquisition of oocyte competence. This is the first report of the localization of crucial proteins for meiosis progression in domestic dogs and identification of the expression and localization of proteins for cell cycle progression performed in this study represented a step of great importance to elucidate the mechanisms involved in the meiosis block in domestic dogs, allowing the advance in this research area. (AU)

• Processo FAPESP: 14/19776-1 - Identificação de marcadores de competência envolvidos no ciclo celular de oócitos caninos pela análise proteômica
• Beneficiário: Maria Denise Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/21667-3 - Identificação de marcadores de competência envolvidos no ciclo celular de oócitos caninos pela análise protêomica
• Beneficiário: Leda Maria Costa Pereira
• Modalidade de apoio: Bolsas no Brasil - Doutorado