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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of EGF on expression and localization of maturation-promoting factor, mitogen-activated protein kinase, p34(cdc2) and cyclin B during different culture periods on in vitro maturation of canine oocytes

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Author(s):
Pereira, Leda Maria C. [1] ; Bersano, Paulo Ricardo O. [2] ; Rocha, Danilo D. [3] ; Lopes, Maria Denise [1]
Total Authors: 4
Affiliation:
[1] FMVZ UNESP, Dept Anim Reprod & Vet Radiol, Botucatu, SP - Brazil
[2] Univ Estadual Ceara, Fac Vet FAVET, Fortaleza, Ceara - Brazil
[3] Univ Fed Ceara, Dept Physiol & Pharmacol, Fortaleza, Ceara - Brazil
Total Affiliations: 3
Document type: Journal article
Source: REPRODUCTION IN DOMESTIC ANIMALS; v. 54, n. 2, p. 325-341, FEB 2019.
Web of Science Citations: 2
Abstract

Contents This study aimed to investigate the localization of MPF, MAPK, p34(cdc2) and cyclin B1 proteins, before and after treatment with EGF during different moments of oocyte maturation. The ovaries obtained from 350 domestic dogs were aseptically isolated, immersed in physiological solution and transported at 4 degrees C. In the laboratory, the ovaries were sectioned for the release of cumulus-oocyte complexes. Cumulus-oocyte complexes were selected and divided into treatment groups with and without EGF and cultured for 24, 48 and 72hr. Immunofluorescence was used for the detection and the localization of MAPK, MPF, p34(cdc2) and cyclin B1 proteins. We observed that the expression and localization of MPF, MAPK, p34(cdc2) and cyclin B1 proteins are associated with meiosis resumption and cell cycle progression, and that EGF influences cell signalling pathways by promoting alterations in the localization of these proteins, improving the acquisition of oocyte competence. This is the first report of the localization of crucial proteins for meiosis progression in domestic dogs and identification of the expression and localization of proteins for cell cycle progression performed in this study represented a step of great importance to elucidate the mechanisms involved in the meiosis block in domestic dogs, allowing the advance in this research area. (AU)

FAPESP's process: 14/19776-1 - Identification of markers of cell cycle racing involved in the canine oocyte proteomic analysis
Grantee:Maria Denise Lopes
Support Opportunities: Regular Research Grants
FAPESP's process: 13/21667-3 - IDENTIFICATION OF MARKERS OF CELL CYCLE COMPETENCE INVOLVED IN THE CANINE OOCYTE PROTEOMIC ANALYSIS
Grantee:Leda Maria Costa Pereira
Support Opportunities: Scholarships in Brazil - Doctorate