Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venom

Autor(es):Mostrar menos -

Fontana Oliveira, Isadora Caruso ^[1] ; de Paula, Marina Oliveira ^[1] ; Boschilha Lastra, Hellen Cristine ^[2] ; Alves, Bruno de Brito ^[3] ; Neto Moreno, Debora Antunes ^[3] ; Yoshida, Edson Hideaki ^[3] ; Amaral Filho, Jorge ^[3] ; Cogo, Jose Carlos ^{[4, 5]} ; Varanda, Eliana Aparecida ^[6] ; Rai, Mahendra ^[7] ; dos Santos, Carolina Alves ^[3] ; Oshima-Franco, Yoko ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sorocaba, UNISO, Vet Med Grad Course, Sorocaba - Brazil

^[2] Univ Sorocaba, UNISO, Bioproc & Biotechnol Engn Course, Sorocaba - Brazil

^[3] Univ Sorocaba, UNISO, Postgrad Program Pharmaceut Sci, Sorocaba, SP - Brazil

^[4] Brazil Univ, Technol & Sci Inst, Bioengn Program, Itaquera, SP - Brazil

^[5] Brazil Univ, Technol & Sci Inst, Biomed Engn Program, Itaquera, SP - Brazil

^[6] Sao Paulo State Univ, UNESP, Pharmaceut Sci Fac Araraquara, Araraquara - Brazil

^[7] SGB Amravati Univ, Dept Biotechnol, Amravati - India

• Tipo de documento: Artigo Científico
• Fonte: DRUG AND CHEMICAL TOXICOLOGY; v. 42, n. 1, p. 60-64, JAN 2 2019.
• Citações Web of Science: 1

Resumo

Nanoparticle-conjugated venom-toxins of venomous animals and its therapeutic efficacy against emerging or neglecting diseases is a promising strategy. In this study, silver nanoparticles (AgNPs similar to 50 nm, 0.081 mg mL(-1)) were studied against the neuromuscular blockade, myotoxic effects induced by Bothrops jararacussu venom (60 mu g mL(-1)) and also against prokaryotic cells. The neurotoxicity was evaluated on ex vivo mouse phrenic nerve-diaphragm using traditional myographic technique, able to obtain functional contractile responses and to check the neurotransmission. The myotoxicity on mammalian cells was evaluated in muscles resulting from pharmacological assays using routine histological techniques and light microscopy. The toxicity to prokaryotic cells was evaluated on Salmonella typhimurium TA100 without metabolic activation. The in vitro preincubation model between AgNPs and venom was enough to abolish toxic effects of B. jararacussu venom, but mammalian cells were highly sensitive to AgNPs more than prokaryotic cells, by acting as dose-independently and dose-dependently parameters, respectively. These results allowed us to conclude that AgNPs showed promising activity as antivenom agent but for its safer use, the toxicity should be evaluated on experimental animals. (AU)

• Processo FAPESP: 08/11005-5 - Dipteryx alata Vogel: estudos da ação antiofídica e de toxicidade
• Beneficiário: Yoko Oshima Franco
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 04/09705-8 - Investigacao farmacologica da atividade antipeconhenta de fracoes obtidas de extratos de casearia sylvestris sw.
• Beneficiário: Yoko Oshima Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/01420-9 - Desenvolvimento e avaliação da eficácia (in vitro e in vivo) de cartucho de hemoperfusor para o tratamento de acidentes ofídicos
• Beneficiário: Yoko Oshima Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/08271-0 - IDENTIFICAÇÃO E FARMACODINÂMICA DE FRAÇÕES NEUROFACILITADORAS DA Casearia sylvestris SW. NA JUNÇÃO NEUROMUSCULAR
• Beneficiário: Yoko Oshima Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/52643-4 - Estudo da capacidade neutralizante do extrato hidroalcoolico de camellia sinensis (cha verde) contra os efeitos neurotoxico e miotoxico da bothropstoxina-i (bothrops jararacussu).
• Beneficiário: Yoko Oshima Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/53883-6 - Dipteryx alata vogel: estudo da acao antiofidica e da toxicidade.
• Beneficiário: Yoko Oshima Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular