Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venom

Author(s):Show less -

Fontana Oliveira, Isadora Caruso ^[1] ; de Paula, Marina Oliveira ^[1] ; Boschilha Lastra, Hellen Cristine ^[2] ; Alves, Bruno de Brito ^[3] ; Neto Moreno, Debora Antunes ^[3] ; Yoshida, Edson Hideaki ^[3] ; Amaral Filho, Jorge ^[3] ; Cogo, Jose Carlos ^{[4, 5]} ; Varanda, Eliana Aparecida ^[6] ; Rai, Mahendra ^[7] ; dos Santos, Carolina Alves ^[3] ; Oshima-Franco, Yoko ^[3]

Affiliation

^[1] Univ Sorocaba, UNISO, Vet Med Grad Course, Sorocaba - Brazil

^[2] Univ Sorocaba, UNISO, Bioproc & Biotechnol Engn Course, Sorocaba - Brazil

^[3] Univ Sorocaba, UNISO, Postgrad Program Pharmaceut Sci, Sorocaba, SP - Brazil

^[4] Brazil Univ, Technol & Sci Inst, Bioengn Program, Itaquera, SP - Brazil

^[5] Brazil Univ, Technol & Sci Inst, Biomed Engn Program, Itaquera, SP - Brazil

^[6] Sao Paulo State Univ, UNESP, Pharmaceut Sci Fac Araraquara, Araraquara - Brazil

^[7] SGB Amravati Univ, Dept Biotechnol, Amravati - India

• Document type: Journal article
• Source: DRUG AND CHEMICAL TOXICOLOGY; v. 42, n. 1, p. 60-64, JAN 2 2019.
• Web of Science Citations: 1

Abstract

Nanoparticle-conjugated venom-toxins of venomous animals and its therapeutic efficacy against emerging or neglecting diseases is a promising strategy. In this study, silver nanoparticles (AgNPs similar to 50 nm, 0.081 mg mL(-1)) were studied against the neuromuscular blockade, myotoxic effects induced by Bothrops jararacussu venom (60 mu g mL(-1)) and also against prokaryotic cells. The neurotoxicity was evaluated on ex vivo mouse phrenic nerve-diaphragm using traditional myographic technique, able to obtain functional contractile responses and to check the neurotransmission. The myotoxicity on mammalian cells was evaluated in muscles resulting from pharmacological assays using routine histological techniques and light microscopy. The toxicity to prokaryotic cells was evaluated on Salmonella typhimurium TA100 without metabolic activation. The in vitro preincubation model between AgNPs and venom was enough to abolish toxic effects of B. jararacussu venom, but mammalian cells were highly sensitive to AgNPs more than prokaryotic cells, by acting as dose-independently and dose-dependently parameters, respectively. These results allowed us to conclude that AgNPs showed promising activity as antivenom agent but for its safer use, the toxicity should be evaluated on experimental animals. (AU)

• FAPESP's process: 08/11005-5 - Dipteryx alata Vogel: antiophidian action and toxicity
• Grantee: Yoko Oshima Franco
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 15/01420-9 - Development and efficacy evaluation (in vitro and in vivo) of a hemoperfuser cartridge for the antiophidian treatment
• Grantee: Yoko Oshima Franco
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/08271-0 - IDENTIFICATION AND PHARMACODYNAMIC OF NEUROFACILITATING FRACTIONS OF Casearia sylvestris SW. AT THE NEUROMUSCULAR JUNCTION
• Grantee: Yoko Oshima Franco
• Support Opportunities: Regular Research Grants