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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Epizootics due to Yellow Fever Virus in Sao Paulo State, Brazil: viral dissemination to new areas (2016-2017)

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Autor(es):
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Cunha, Mariana Sequetin [1] ; da Costa, Antonio Charlys [2] ; Couto de Azevedo Fernandes, Natalia Coelho [3] ; Guerra, Juliana Mariotti [3] ; Pereira dos Santos, Fabiana Cristina [1] ; Nogueira, Juliana Silva [1] ; D'Agostino, Leandro Guariglia [1] ; Komninakis, Shirley Vasconcelos [4, 5] ; Witkin, Steven S. [2, 6] ; Ressio, Rodrigo Albergaria [3] ; Maeda, Adriana Yurika [1] ; Silva Vasami, Fernanda Gisele [1] ; Abreu Kaigawa, Ursula Mitsue [1] ; de Azevedo, Lais Sampaio [1] ; de Souza Facioli, Paloma Alana [1] ; Lima Macedo, Fernando Luiz [1] ; Sabino, Ester Cerdeira [2] ; Leal, Elcio [7] ; de Souza, Renato Pereira [1]
Número total de Autores: 19
Afiliação do(s) autor(es):
[1] Adolfo Lutz Inst, Virol Ctr, Vector Borne Dis Grp, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Trop Med, Sao Paulo - Brazil
[3] Adolfo Lutz Inst, Pathol Ctr, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Lab Retrovirol, Sao Paulo - Brazil
[5] FMABC, Postgrad Program Hlth Sci, Santo Andre - Brazil
[6] Weill Cornell Med, Dept Obstet & Gynecol, New York, NY - USA
[7] Fed Univ Para, Inst Biol Sci, Belem, Para - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 9, APR 2 2019.
Citações Web of Science: 1
Resumo

Beginning in late 2016 Brazil faced the worst outbreak of Yellow Fever in recent decades, mainly located in southeastern rural regions of the country. In the present study we characterize the Yellow Fever Virus (YFV) associated with this outbreak in Sao Paulo State, Brazil. Blood or tissues collected from 430 dead monkeys and 1030 pools containing a total of 5,518 mosquitoes were tested for YFV by quantitative RT-PCR, immunohistochemistry (IHC) and indirect immunofluorescence. A total of 67 monkeys were YFV-positive and 3 pools yielded YFV following culture in a C6/36 cell line. Analysis of five nearly full length genomes of YFV from collected samples was consistent with evidence that the virus associated with the Sao Paulo outbreak originated in Minas Gerais. The phylogenetic analysis also showed that strains involved in the 2016-2017 outbreak in distinct Brazilian states (i.e., Minas Gerais, Rio de Janeiro, Espirito Santo) intermingled in maximum-likelihood and Bayesian trees. Conversely, the strains detected in Sao Paulo formed a monophyletic cluster, suggesting that they were local-adapted. The finding of YFV by RT-PCR in five Callithrix monkeys who were all YFV-negative by histopathology or immunohistochemistry suggests that this YFV lineage circulating in Sao Paulo is associated with different outcomes in Callithrix when compared to other monkeys. (AU)

Processo FAPESP: 17/00021-9 - Metagenômica viral da dengue, Chikungunya e Zika: acompanhar, explicar e prever a transmissão e distribuição espaço-temporal no Brasil
Beneficiário:Antonio Charlys da Costa
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/01735-2 - Metagenômica viral de dengue, Chikungunya e Zika vírus: acompanhar, explicar e prever a transmissão e distribuição espaço-temporal no Brasil
Beneficiário:Ester Cerdeira Sabino
Linha de fomento: Auxílio à Pesquisa - Regular