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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Epizootics due to Yellow Fever Virus in Sao Paulo State, Brazil: viral dissemination to new areas (2016-2017)

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Cunha, Mariana Sequetin [1] ; da Costa, Antonio Charlys [2] ; Couto de Azevedo Fernandes, Natalia Coelho [3] ; Guerra, Juliana Mariotti [3] ; Pereira dos Santos, Fabiana Cristina [1] ; Nogueira, Juliana Silva [1] ; D'Agostino, Leandro Guariglia [1] ; Komninakis, Shirley Vasconcelos [4, 5] ; Witkin, Steven S. [2, 6] ; Ressio, Rodrigo Albergaria [3] ; Maeda, Adriana Yurika [1] ; Silva Vasami, Fernanda Gisele [1] ; Abreu Kaigawa, Ursula Mitsue [1] ; de Azevedo, Lais Sampaio [1] ; de Souza Facioli, Paloma Alana [1] ; Lima Macedo, Fernando Luiz [1] ; Sabino, Ester Cerdeira [2] ; Leal, Elcio [7] ; de Souza, Renato Pereira [1]
Total Authors: 19
[1] Adolfo Lutz Inst, Virol Ctr, Vector Borne Dis Grp, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Trop Med, Sao Paulo - Brazil
[3] Adolfo Lutz Inst, Pathol Ctr, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Lab Retrovirol, Sao Paulo - Brazil
[5] FMABC, Postgrad Program Hlth Sci, Santo Andre - Brazil
[6] Weill Cornell Med, Dept Obstet & Gynecol, New York, NY - USA
[7] Fed Univ Para, Inst Biol Sci, Belem, Para - Brazil
Total Affiliations: 7
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, APR 2 2019.
Web of Science Citations: 1

Beginning in late 2016 Brazil faced the worst outbreak of Yellow Fever in recent decades, mainly located in southeastern rural regions of the country. In the present study we characterize the Yellow Fever Virus (YFV) associated with this outbreak in Sao Paulo State, Brazil. Blood or tissues collected from 430 dead monkeys and 1030 pools containing a total of 5,518 mosquitoes were tested for YFV by quantitative RT-PCR, immunohistochemistry (IHC) and indirect immunofluorescence. A total of 67 monkeys were YFV-positive and 3 pools yielded YFV following culture in a C6/36 cell line. Analysis of five nearly full length genomes of YFV from collected samples was consistent with evidence that the virus associated with the Sao Paulo outbreak originated in Minas Gerais. The phylogenetic analysis also showed that strains involved in the 2016-2017 outbreak in distinct Brazilian states (i.e., Minas Gerais, Rio de Janeiro, Espirito Santo) intermingled in maximum-likelihood and Bayesian trees. Conversely, the strains detected in Sao Paulo formed a monophyletic cluster, suggesting that they were local-adapted. The finding of YFV by RT-PCR in five Callithrix monkeys who were all YFV-negative by histopathology or immunohistochemistry suggests that this YFV lineage circulating in Sao Paulo is associated with different outcomes in Callithrix when compared to other monkeys. (AU)

FAPESP's process: 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
Grantee:Antonio Charlys da Costa
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/01735-2 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
Grantee:Ester Cerdeira Sabino
Support type: Regular Research Grants