Influence of Leishmania (Viannia) braziliensis infection on the attractiveness of BALB/c mice to Nyssomyia neivai (Diptera: Psychodidae)

Autor(es)

da Rocha Silva, Flavia Benini ^[1] ; Miguel, Danilo Ciccone ^[2] ; Machado, Vicente Estevam ^[1] ; Cruz Oliveira, Wanderson Henrique ^[1] ; Goulart, Thais Marchi ^[2] ; Tosta, Christiann Davis ^[3] ; Pinheiro, Hildete Prisco ^[4] ; Pinto, Mara Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Fac Ciencias Farmaceut, Dept Ciencias Biol, Campus Araraquara, Araraquara, SP - Brazil

^[2] Univ Estadual Campinas UNICAMP, Dept Biol Anim, Campinas, SP - Brazil

^[3] Inst Fed Educ Ciencia & Tecnol Sao Paulo IFSP, Campus Matao, Matao, SP - Brazil

^[4] Univ Estadual Campinas UNICAMP, Dept Estat, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 14, n. 4 APR 1 2019.
• Citações Web of Science: 0

Resumo

Background Phlebotomine sand flies are vectors for several pathogens, with Leishmania being the most important. In Brazil, the main aetiological agent of American cutaneous leishmaniasis (ACL) is Leishmania (Viannia) braziliensis, and Nyssomyia neivai is one of its main vectors in Sao Paulo state and other areas of South America. Similar to other haematophagous insects, sand flies use volatile compounds called kairomones to locate their hosts for blood meals. A possible increase in the attractiveness of hosts infected with Leishmania infantum to their vectors has been demonstrated. In the present study, we aimed to investigate whether L. braziliensis-infected hosts present higher attractiveness to Ny. neivai and to identify differences in the volatile compounds released by infected and uninfected mice. Results Behavioural experiments in which sand fly females directly fed on infected or uninfected mice showed no significant differences in the attractiveness of the mice or the blood volume ingested. Y-tube olfactometer bioassays also revealed no significant differences in the attractiveness of these hosts to Ny. neivai. No differences were observed in the profiles of the volatile compounds released by the two groups of mice. However, PCA and cluster analysis were able to classify the 31 identified compounds into three clusters according to their abundances. This classification showed a possible role for individual variation in the absence of differences in volatile profiles and attractiveness between infected and uninfected mice. Conclusion In this first cross-sectional study with an aetiological agent of ACL, there were no statistically significant differences in the attractiveness of infected hosts to their vector. (AU)

• Processo FAPESP: 12/23832-9 - Aspectos biológicos de Nyssomyia neivai (Diptera: Psychodidae) e aprimoramento das técnicas de colonização em laboratório
• Beneficiário: Mara Cristina Pinto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/21129-4 - O papel das proteínas ligantes de ácidos graxos na infecção de macrófagos por Leishmania: um alvo potencial para novas drogas contra leishmaniose
• Beneficiário: Danilo Ciccone Miguel
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 15/21100-9 - Investigação sobre a atratividade de hospedeiros infectados com Leishmania (Viannia) braziliensis (Kinetoplastida: Trypanosomatidae) para Nyssomyia neivai (Diptera: Psychodidae)
• Beneficiário: Flávia Benini da Rocha Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado