Second-harmonic generation imaging analysis can help distinguish sarcoidosis from tuberculoid leprosy

Autor(es)

Utino, Fabiane Leonel ^{[1, 2]} ; Garcia, Marina ^[1] ; Neves Ferreira Velho, Paulo Eduardo ^[2] ; Eloy da Costa Franca, Andrea Fernandes ^[2] ; Stelini, Rafael Fantelli ^[1] ; Pelegati, Vitor Bianchin ^{[3, 4]} ; Cesar, Carlos Lenz ^{[3, 5, 4]} ; de Souza, Elemir Macedo ^[2] ; Cintra, Maria Leticia ^[1] ; Damiani, Gislaine Vieira ^{[4, 6]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Pathol, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Dept Dermatol, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Gleb Wataghin Inst Phys, Campinas, SP - Brazil

^[4] Technol Photon Appl Cell Biol, Campinas, SP - Brazil

^[5] Univ Fed Ceara, Dept Phys, Fortaleza, Ceara - Brazil

^[6] Fed Inst Educ Sci & Technol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biomedical Optics; v. 23, n. 12 DEC 2018.
• Citações Web of Science: 0

Resumo

Sarcoidosis and tuberculoid leprosy (TL) are prototypes of granulomatous inflammation in dermatology, which embody one of the histopathology limitations in distinguishing some diseases. Recent advances in the use of nonlinear optical microscopy in skin have enabled techniques, such as second-harmonic generation (SHG), to become powerful tools to study the physical and biochemical properties of skin. We use SHG images to analyze the collagen network, to distinguish differences between sarcoidosis and TL granulomas. SHG images obtained from skin biopsies of 33 patients with TL and 24 with sarcoidosis retrospectively were analyzed using first-order statistics (FOS) and second-order statistics, such as gray-level co-occurrence matrix (GLCM). Among the four parameters evaluated (optical density, entropy, contrast, and second angular moment), only contrast demonstrated statistical significance, being higher in sarcoidosis (p = 0.02; 4908.31 versus 2822.17). The results may indicate insufficient differentiating power for most tested FOS and GLCM parameters in classifying sarcoidosis and TL granulomas, when used individually. But in combination with histopathology (H\&E and complementary stains, such as silver and fast acid stains), SHG analysis, like contrast, can contribute to distinguishing between these diseases. This study can provide a way to evaluate collagen distribution in granulomatous diseases. (c) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. (AU)

• Processo FAPESP: 17/17149-8 - Perfil imunofenotípico do infiltrado macrofágico na doença de jorge lobo e hanseníase virchowiana - análise comparativa
• Beneficiário: Maria Letícia Cintra
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/22927-7 - Análise comparativa da distribuição das fibras reticulínicas e colágenas na pele de pacientes com sarcoidose e hanseníase tuberculoide
• Beneficiário: Maria Letícia Cintra
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/50938-8 - INCT 2014: em Fotônica Aplicada à Biologia Celular INFABiC
• Beneficiário: Hernandes Faustino de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/51959-0 - Biologia das doenças neoplásicas da medula óssea
• Beneficiário: Sara Teresinha Olalla Saad
• Modalidade de apoio: Auxílio à Pesquisa - Temático