Screening and optimizing fermentation production of l-asparaginase by Aspergillus terreus strain S-18 isolated from the Brazilian Caatinga Biome

Autor(es)

da Rocha, W. R. V. ^[1] ; Costa-Silva, T. A. ^[2] ; Agamez-Montalvo, G. S. ^[3] ; Feitosa, V. A. ^[2] ; Machado, S. E. F. ^[4] ; de Souza Lima, G. M. ^[4] ; Pessoa-, Jr., A. ^[2] ; Alves, H. S. ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Paraiba, Campina Grande, PB - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci, Ave Prof Lineu Prestes 580, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Fed Ceara, Fortaleza, Ceara - Brazil

^[4] Univ Fed Pernambuco, Recife, PE - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Microbiology; v. 126, n. 5, p. 1426-1437, MAY 2019.
• Citações Web of Science: 0

Resumo

Aims The aim of this study was to find new eukaryotic sources of the l-asparaginase (l-ASNase), since the prokaryotic sources of the enzyme are well-reported as causing allergic hypersensitivity reactions in a significant number of patients. This report describes screening for l-ASNase production by filamentous fungi isolated from the Brazilian Caatinga, and the optimization of fermentation parameters to increase fungal growth and improve yield in the production of l-ASNase. Methods and Results Thirty-two filamentous fungi were investigated in this study. When Aspergillus terreus strain S-18 was cultured in a proline-enriched medium, intracellular l-ASNase was expressed in concurrence with reduced l-glutaminase (l-GLUase) and protease activities. Fermentation conditions were then optimized in a 5-l bioreactor system to produce a maximum volumetric yield of 108 U total of l-ASNase activity. Conclusions The work reported here represents the first attempt to produce l-ASNase by filamentous fungi isolated from Brazil and offers a promising alternative eukaryotic source for l-ASNase production. Significance and Impact of the study In order to minimize the side effects caused by bacterial l-ASNase, the search of eukaryotic micro-organism for l-ASNase was carried out in fungi. This study demonstrates the diversity of filamentous fungi isolated from the Brazilian Caatinga Biome and the importance of knowledge of the microbial metabolism to obtain high concentrations of biotechnological products. (AU)

• Processo FAPESP: 13/19584-2 - Produção de L-asparaginase extracelular pelo fungo Arpergillus terreus
• Beneficiário: Tales Alexandre da Costa e Silva
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/08617-7 - Produção de L-asparaginase extracelular: da bioprospecção à engenharia de um biofármaco antileucêmico
• Beneficiário: Adalberto Pessoa Junior
• Linha de fomento: Auxílio à Pesquisa - Temático