Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Screening and optimizing fermentation production of l-asparaginase by Aspergillus terreus strain S-18 isolated from the Brazilian Caatinga Biome

Full text
Author(s):
da Rocha, W. R. V. [1] ; Costa-Silva, T. A. [2] ; Agamez-Montalvo, G. S. [3] ; Feitosa, V. A. [2] ; Machado, S. E. F. [4] ; de Souza Lima, G. M. [4] ; Pessoa-, Jr., A. [2] ; Alves, H. S. [1]
Total Authors: 8
Affiliation:
[1] State Univ Paraiba, Campina Grande, PB - Brazil
[2] Univ Sao Paulo, Fac Pharmaceut Sci, Ave Prof Lineu Prestes 580, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Fed Ceara, Fortaleza, Ceara - Brazil
[4] Univ Fed Pernambuco, Recife, PE - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Applied Microbiology; v. 126, n. 5, p. 1426-1437, MAY 2019.
Web of Science Citations: 0
Abstract

Aims The aim of this study was to find new eukaryotic sources of the l-asparaginase (l-ASNase), since the prokaryotic sources of the enzyme are well-reported as causing allergic hypersensitivity reactions in a significant number of patients. This report describes screening for l-ASNase production by filamentous fungi isolated from the Brazilian Caatinga, and the optimization of fermentation parameters to increase fungal growth and improve yield in the production of l-ASNase. Methods and Results Thirty-two filamentous fungi were investigated in this study. When Aspergillus terreus strain S-18 was cultured in a proline-enriched medium, intracellular l-ASNase was expressed in concurrence with reduced l-glutaminase (l-GLUase) and protease activities. Fermentation conditions were then optimized in a 5-l bioreactor system to produce a maximum volumetric yield of 108 U total of l-ASNase activity. Conclusions The work reported here represents the first attempt to produce l-ASNase by filamentous fungi isolated from Brazil and offers a promising alternative eukaryotic source for l-ASNase production. Significance and Impact of the study In order to minimize the side effects caused by bacterial l-ASNase, the search of eukaryotic micro-organism for l-ASNase was carried out in fungi. This study demonstrates the diversity of filamentous fungi isolated from the Brazilian Caatinga Biome and the importance of knowledge of the microbial metabolism to obtain high concentrations of biotechnological products. (AU)

FAPESP's process: 13/19584-2 - Production of extracellular L-asparaginase by fungus Aspergullus terreus
Grantee:Tales Alexandre da Costa e Silva
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/08617-7 - Production of extracellular L-asparaginase: from bioprospecting to the engineering of an antileukemic biopharmaceutical
Grantee:Adalberto Pessoa Junior
Support Opportunities: Research Projects - Thematic Grants