Hyperthermia-induced seizures followed by repetitive stress are associated with age-dependent changes in specific aspects of the mouse stress system

Autor(es)

Umeoka, Eduardo H. L. ^{[1, 2]} ; Robinson, Edward J. ^{[1, 3]} ; Turimella, Sada Lakshmi ^[1] ; van Campen, Jolien S. ^{[1, 4]} ; Motta-Teixeira, Livia C. ^[5] ; Sarabdjitsingh, R. Angela ^[1] ; Garcia-Cairasco, Norberto ^{[2, 6]} ; Braun, Kees ^[4] ; de Graan, Pierre N. ^[1] ; Joels, Marian ^{[1, 7]}

Afiliação do(s) autor(es)

^[1] Univ Utrecht, Dept Translat Neurosci, Brain Ctr Rudolf Magnus, Univ Med Ctr Utrecht, Utrecht - Netherlands

^[2] Univ Sao Paulo, Neurosci & Behav Sci Dept, Ribeirao Preto Sch Med, Ribeirao Preto, SP - Brazil

^[3] Univ Amsterdam, Swammerdam Inst Life Sci, Amsterdam - Netherlands

^[4] Univ Med Ctr Utrecht, Dept Pediat Neurol, Brain Ctr Rudolf Magnus, Utrecht - Netherlands

^[5] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Physiol Dept, Ribeirao Preto Sch Med, Ribeirao Preto, SP - Brazil

^[7] Univ Groningen, Univ Med Ctr Groningen, Groningen - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: Journal of Neuroendocrinology; v. 31, n. 4 APR 2019.
• Citações Web of Science: 1

Resumo

Stress is among the most frequently self-reported factors provoking epileptic seizures in children and adults. It is still unclear, however, why some people display stress-sensitive seizures and others do not. Recently, we showed that young epilepsy patients with stress-sensitive seizures exhibit a dysregulated hypothalamic-pituitary-adrenal (HPA)-axis. Most likely, this dysregulation gradually develops, and is triggered by stressors occurring early in life (early-life stress {[}ELS]). ELS may be particularly impactful when overlapping with the period of epileptogenesis. To examine this in a controlled and prospective manner, the present study investigated the effect of repetitive variable stressors or control treatment between postnatal day (PND) 12 and 24 in male mice exposed on PND10 to hyperthermia (HT)-induced prolonged seizures (control: normothermia). A number of peripheral and central indices of HPA-axis activity were evaluated at pre-adolescent and young adult age (ie, at PND25 and 90, respectively). At PND25 but not at PND90, body weight gain and absolute as well as relative (to body weight) thymus weight were reduced by ELS (vs control), whereas relative adrenal weight was enhanced, confirming the effectiveness of the stress treatment. Basal and stress-induced corticosterone levels were unaffected, though, by ELS at both ages. HT by itself did not affect any of these peripheral markers of HPA-axis activity, nor did it interact with ELS. However, centrally we did observe age-specific interaction effects of HT and ELS with regard to hippocampal glucocorticoid receptor mRNA expression, neurogenesis with the immature neurone marker doublecortin and the number of hilar (ectopic) granule cells using Prox1 staining. This lends some support to the notion that exposure to repetitive stress after HT-induced seizures may dysregulate central components of the stress system in an age-dependent manner. Such dysregulation could be one of the mechanisms conferring higher vulnerability of individuals with epilepsy to develop seizures in the face of stress. (AU)

• Processo FAPESP: 16/18941-4 - Efeitos da ausência da melatonina maternal durante a gestação e lactação sobre o sistema neuroimune da prole.
• Beneficiário: Lívia Clemente Motta Teixeira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 11/13412-0 - Caracterização de aspectos comportamentais e morfológicos associados à atividade do eixo hipotálamo-hipófise-adrenal (HHA) em ratos wistar audiogênicos após crises crônicas
• Beneficiário: Eduardo Henrique de Lima Umeoka
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/21401-0 - Efeito do estresse múltiplo na vida precoce sobre a epileptogênese induzida por hipertermia em camundongos adolescentes e adultos
• Beneficiário: Eduardo Henrique de Lima Umeoka
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado