beta-carotene and -tocopherol coencapsulated in nanostructured lipid carriers of murumuru (Astrocaryum murumuru) butter produced by phase inversion temperature method: characterisation, dynamic in vitro digestion and cell viability study

Autor(es)

Gomes, G. V. L. ^[1] ; Sola, M. R. ^[1] ; Rochetti, A. L. ^[2] ; Fukumasu, H. ^[2] ; Vicente, A. A. ^[3] ; Pinho, S. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Food Engn, Pirassununga - Brazil

^[2] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Vet Med, Pirassununga - Brazil

^[3] Univ Minho, Ctr Biol Engn, CEB, Campus Gualtar, Braga - Portugal

• Tipo de documento: Artigo Científico
• Fonte: Journal of Microencapsulation; v. 36, n. 1, p. 43-52, JAN 2 2019.
• Citações Web of Science: 1

Resumo

Hydrophobic bioactives can be more easily incorporated into food and have their bioavailability enhanced if nanostructured lipid carriers (NLC) are used as carriers. In the present study, beta-carotene-loaded NLC were produced by low emulsification using murumuru butter and a mixture of Span 80 and Cremophor RH40 as surfactants. Their average diameter was 35nm and alpha-tocopherol was required to protect the encapsulated -carotene. Besides the evaluation of their physicochemical stability, NLC were submitted to dynamic in vitro digestion and cell viability assays with Caco-2 and HEPG cells. The bioaccessibility of beta-carotene in the dynamic system was about 42%. Regarding cell viability, results indicated NLC were toxic to the cell cultures tested. Such high toxicity is probably related to the type of surfactant used and to the extremely reduced particle size, which may have led to an intense and fast permeation of the NLC through the cells. (AU)

• Processo FAPESP: 11/01260-0 - Estudo de viabilidade da produção de Nanoparticulas Lipídicas Sólidas pelo método da temperatura de transição de fases (método PIT)
• Beneficiário: Mirella Roberta Sola
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/18495-3 - Digestibilidade in vitro dinâmica de nanopartículas lipídicas sólidas encapsulando beta-caroteno
• Beneficiário: Graziela Veiga de Lara Gomes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 10/20523-0 - Nanopartículas lipídicas sólidas produzidas por método de baixa energia: otimização das condições de processo, avaliação da absorção de bioativo encapsulado e estudo de digestibilidade in vitro
• Beneficiário: Graziela Veiga de Lara Gomes
• Modalidade de apoio: Bolsas no Brasil - Doutorado