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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

beta-carotene and -tocopherol coencapsulated in nanostructured lipid carriers of murumuru (Astrocaryum murumuru) butter produced by phase inversion temperature method: characterisation, dynamic in vitro digestion and cell viability study

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Gomes, G. V. L. [1] ; Sola, M. R. [1] ; Rochetti, A. L. [2] ; Fukumasu, H. [2] ; Vicente, A. A. [3] ; Pinho, S. C. [1]
Total Authors: 6
[1] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Food Engn, Pirassununga - Brazil
[2] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Vet Med, Pirassununga - Brazil
[3] Univ Minho, Ctr Biol Engn, CEB, Campus Gualtar, Braga - Portugal
Total Affiliations: 3
Document type: Journal article
Source: Journal of Microencapsulation; v. 36, n. 1, p. 43-52, JAN 2 2019.
Web of Science Citations: 1

Hydrophobic bioactives can be more easily incorporated into food and have their bioavailability enhanced if nanostructured lipid carriers (NLC) are used as carriers. In the present study, beta-carotene-loaded NLC were produced by low emulsification using murumuru butter and a mixture of Span 80 and Cremophor RH40 as surfactants. Their average diameter was 35nm and alpha-tocopherol was required to protect the encapsulated -carotene. Besides the evaluation of their physicochemical stability, NLC were submitted to dynamic in vitro digestion and cell viability assays with Caco-2 and HEPG cells. The bioaccessibility of beta-carotene in the dynamic system was about 42%. Regarding cell viability, results indicated NLC were toxic to the cell cultures tested. Such high toxicity is probably related to the type of surfactant used and to the extremely reduced particle size, which may have led to an intense and fast permeation of the NLC through the cells. (AU)

FAPESP's process: 10/20523-0 - Solid lipid nanoparticles production by low-energy method: process optimization, evaluation of bioactive absorption and in vitro digestibility
Grantee:Graziela Veiga de Lara Gomes
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/18495-3 - In vitro dynamic digestibility of solid lipid nanoparticles encapsulating beta-carotene
Grantee:Graziela Veiga de Lara Gomes
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 11/01260-0 - Feasibility of producing solid lipid nanoparticles by phase inversion temperature method (PIT method)
Grantee:Mirella Roberta Sola
Support type: Scholarships in Brazil - Scientific Initiation