Development of nanostructured lipid carrier of Illipe butter and Calendula oil as a quercetin encapsulation system: physico-chemical and biological evaluation

• Grant number: 13/24413-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2014
• End date: May 31, 2017
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Priscyla Daniely Marcato Gaspari
• Grantee: Thais Priscilla Pivetta
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 16/07596-4 - Encapsulation of thymol in nanostructured lipid carriers composed of natural lipids, BE.EP.IC

Abstract

Products of degradation metabolism and the own cellular respiration can generate oxidative stress which may lead peroxidation of fat acids on the lipid layer, process which leading to the cellular death and skin aging. Thereby, there is a search for antioxidant molecules that can control these effects, such as quercetin. Quercetin is a flavonoid that has antioxidant properties, thus it's used to eliminate reactive oxygen species and inhibit peroxidation, process that are related to the senescence body. However, quercetin has low stability and skin permeation. Thus, its incorporation in particulate systems is necessary in order to quercetin become more effective to clinical application. So, the aim of this project is the development of nanostructured lipid carrier composed by natural lipids of Illipe butter and Calendula oil as an encapsulation system of quercetin; aiming to increase cutaneous permeation and protect the active of photodegradation. Furthermore, the anti-inflamatory, anti-sseptic, antioxidant and emolient properties of the chosen lipids could be added to the quercetin antioxidant activity. The NLCs will be prepared by the microemulsion and sonication method and will be characterized regarding their morphology by atomic force microscopy, diameter and surface charge by photon correlation spectroscopy. Furthermore, the stability and encapsulation efficiency of the formulation will be evaluated. Posteriorly, the antioxidant activity of quercetin loaded on nanostructured lipid carrier will be determinated and compared with free quercetin. Still will be performed in vitro study to evaluate the allergenic potencial of the formulation and the evaluation of cutaneous permeation. It is expected that NLCs increase the stability and the skin permeation of quercetin.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PIVETTA, THAIS P.; SIMOES, SANDRA; ARAUJO, MARGARETE M.; CARVALHO, TANIA; ARRUDA, CAROLINE; MARCATO, PRISCYLA D.. Development of nanoparticles from natural lipids for topical delivery of thymol: Investigation of its anti-inflammatory properties. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 164, p. 281-290, APR 1 2018. (13/24413-2, 16/07596-4)

PIVETTA, THAIS P.; SILVA, LETICIA B.; KAWAKAMI, CAMILA M.; ARAUJO, MARGARETE M.; DEL LAMA, MARIA PERPETUA F. M.; NAAL, ROSE MARY Z. G.; MARIA-ENGLER, SILVYA S.; GASPAR, LORENA R.; MARCATO, PRISCYLA D.. Topical formulation of quercetin encapsulated in natural lipid nanocarriers: Evaluation of biological properties and phototoxic effect. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v. 53, OCT 2019. (16/07596-4, 14/50928-2, 13/24413-2, 08/57805-2)