Products of degradation metabolism and the own cellular respiration can generate oxidative stress which may lead peroxidation of fat acids on the lipid layer, process which leading to the cellular death and skin aging. Thereby, there is a search for antioxidant molecules that can control these effects, such as quercetin. Quercetin is a flavonoid that has antioxidant properties, thus it's used to eliminate reactive oxygen species and inhibit peroxidation, process that are related to the senescence body. However, quercetin has low stability and skin permeation. Thus, its incorporation in particulate systems is necessary in order to quercetin become more effective to clinical application. So, the aim of this project is the development of nanostructured lipid carrier composed by natural lipids of Illipe butter and Calendula oil as an encapsulation system of quercetin; aiming to increase cutaneous permeation and protect the active of photodegradation. Furthermore, the anti-inflamatory, anti-sseptic, antioxidant and emolient properties of the chosen lipids could be added to the quercetin antioxidant activity. The NLCs will be prepared by the microemulsion and sonication method and will be characterized regarding their morphology by atomic force microscopy, diameter and surface charge by photon correlation spectroscopy. Furthermore, the stability and encapsulation efficiency of the formulation will be evaluated. Posteriorly, the antioxidant activity of quercetin loaded on nanostructured lipid carrier will be determinated and compared with free quercetin. Still will be performed in vitro study to evaluate the allergenic potencial of the formulation and the evaluation of cutaneous permeation. It is expected that NLCs increase the stability and the skin permeation of quercetin.
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