An eco-friendly sample preparation procedure base on low-density solvent-based air-assisted liquid-liquid microextraction for the simultaneous determination of 21 potential endocrine disruptors in urine samples by liquid chromatography-tandem mass spectrometry

Autor(es)

Rocha, Bruno Alves ^{[1, 2]} ; de Oliveira, Silvana Ruella ^[2] ; da Silva, Rodrigo Moreira ^[3] ; Mazzaron Barcelos, Gustavo Rafael ^[4] ; Moraes de Oliveira, Anderson Rodrigo ^[5] ; Barbosa Jr, Fernando

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Rua Prof Artur Riedel 275, BR-09972270 Diadema, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Lab Toxicol & Essencialidade Metais, Ave Cafe S-N, BR-14049903 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS, BR-14040903 Ribeirao Preto, SP - Brazil

^[4] Univ Fed Sao Paulo, Inst Hlth & Soc, Dept Biosci, Ave Ana Costa 95, BR-11060001 Santos, SP - Brazil

^[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14049901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microchemical Journal; v. 147, p. 207-214, JUN 2019.
• Citações Web of Science: 1

Resumo

A simple and rapid procedure for simultaneous determination of 21 potential endocrine-disrupting chemicals (EDs) in urine samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. The novelty of the present procedure is based on the use of low-density solvent air-assisted liquid-liquid microextraction (LDS-AALLME) as a sample preparation step. The target analytes were 7 bisphenols, 7 parabens, 5 benzophenones, and two antimicrobials. The parameters (type and volume of solvent, extraction time (cycles), sample pH, ionic strength, and agitation) that influence the extraction efficiency in the LDS-AALLME were investigated and optimized. Under the optimized conditions, the matrix-matched calibration curves of all analytes presented correlation coefficients higher than 0.99 (range level of 1-20 ng mL(-1)). Very low LODs and LOQs ranging in 0.0003-0.15 ng mL(-1) and 0.001-0.2 ng mL(-1), respectively, were obtained. Recoveries from spiked samples at two concentrations (2.50 ng mL(-1) and 15 ng mL(-1)) ranged from 91% to 120% while the precision (%, RSD) was lower than 15% for all studied compounds. The proposed combination of LDS-AALLME with LC-MS/MS is an environmentally friendly, sensitive and simple analytical alternative for the determination of EDs in urine samples without the use of any special apparatus for sample preparation, which is particularly suitable for human biomonitoring studies where many samples should be processed in less time. Finally, the proposed procedure was successfully applied to the determination of the analytes in 20 urine samples from Brazilians adults. (AU)

• Processo FAPESP: 14/50945-4 - INCT 2014: Instituto Nacional de Tecnologias Alternativas para Detecção, Avaliação Toxicológica e Remoção de Micropoluentes e Radioativos
• Beneficiário: Maria Valnice Boldrin
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/07534-4 - Desenvolvimento de métodos cromatográficos/eletroforéticos para posterior aplicação em estudos in vitro de inibição enzimática e predição de interações medicamentosas de praguicidas quirais - Fase 2
• Beneficiário: Anderson Rodrigo Moraes de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/19914-5 - Impacto da exposição transplacentária ao fumo do tabaco no DNA do recém-nascido: avaliação de dano genético e alterações epigenéticas
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular