An eco-friendly sample preparation procedure base on low-density solvent-based air-assisted liquid-liquid microextraction for the simultaneous determination of 21 potential endocrine disruptors in urine samples by liquid chromatography-tandem mass spectrometry

Author(s)

Rocha, Bruno Alves ^{[1, 2]} ; de Oliveira, Silvana Ruella ^[2] ; da Silva, Rodrigo Moreira ^[3] ; Mazzaron Barcelos, Gustavo Rafael ^[4] ; Moraes de Oliveira, Anderson Rodrigo ^[5] ; Barbosa Jr, Fernando

Affiliation

^[1] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Rua Prof Artur Riedel 275, BR-09972270 Diadema, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Lab Toxicol & Essencialidade Metais, Ave Cafe S-N, BR-14049903 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS, BR-14040903 Ribeirao Preto, SP - Brazil

^[4] Univ Fed Sao Paulo, Inst Hlth & Soc, Dept Biosci, Ave Ana Costa 95, BR-11060001 Santos, SP - Brazil

^[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14049901 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Microchemical Journal; v. 147, p. 207-214, JUN 2019.
• Web of Science Citations: 1

Abstract

A simple and rapid procedure for simultaneous determination of 21 potential endocrine-disrupting chemicals (EDs) in urine samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. The novelty of the present procedure is based on the use of low-density solvent air-assisted liquid-liquid microextraction (LDS-AALLME) as a sample preparation step. The target analytes were 7 bisphenols, 7 parabens, 5 benzophenones, and two antimicrobials. The parameters (type and volume of solvent, extraction time (cycles), sample pH, ionic strength, and agitation) that influence the extraction efficiency in the LDS-AALLME were investigated and optimized. Under the optimized conditions, the matrix-matched calibration curves of all analytes presented correlation coefficients higher than 0.99 (range level of 1-20 ng mL(-1)). Very low LODs and LOQs ranging in 0.0003-0.15 ng mL(-1) and 0.001-0.2 ng mL(-1), respectively, were obtained. Recoveries from spiked samples at two concentrations (2.50 ng mL(-1) and 15 ng mL(-1)) ranged from 91% to 120% while the precision (%, RSD) was lower than 15% for all studied compounds. The proposed combination of LDS-AALLME with LC-MS/MS is an environmentally friendly, sensitive and simple analytical alternative for the determination of EDs in urine samples without the use of any special apparatus for sample preparation, which is particularly suitable for human biomonitoring studies where many samples should be processed in less time. Finally, the proposed procedure was successfully applied to the determination of the analytes in 20 urine samples from Brazilians adults. (AU)

• FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
• Grantee: Maria Valnice Boldrin
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/07534-4 - Development of chromatographic / electrophoretic methods to be further applied in in vitro enzymatic inhibition studies and prediction of drug interactions of chiral pesticides - Phase 2
• Grantee: Anderson Rodrigo Moraes de Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/19914-5 - Impact of transplacental exposure to tobacco smoke in the DNA of newborn: genetic and epigenetic effects
• Grantee: Fernando Barbosa Júnior
• Support Opportunities: Regular Research Grants