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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Revisiting SEPT7 and the slippage of beta-strands in the septin family

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Autor(es):
Brognara, Gabriel [1] ; Pereira, Humberto D'Muniz [1] ; Brandao-Neto, Jose [2] ; Ulian Araujo, Ana Paula [1] ; Garratt, Richard Charles [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sao Carlos Inst Phys, Ave Joao Dagnone 1100, BR-13563120 Sao Carlos, SP - Brazil
[2] Diamond Light Source, Harwell Sci & Innovat Campus, Didcot, Oxon - England
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Journal of Structural Biology; v. 207, n. 1, p. 67-73, JUL 1 2019.
Citações Web of Science: 0
Resumo

Septins are GTP-binding proteins that will often spontaneously assemble into filaments. In some species, particularly budding yeast, it is well known that these are capable of associating with membranes in order to fulfill their cellular role as a component of the cytoskeleton. Different from other human septins, SEPT7 appears to be unique in that it is an essential component of all hetero-oligomeric complexes described to date. As a step towards understanding the molecular basis of filament assembly, here we present two high-resolution structures of the SEPT7 GTPase domain complexed with GDP. One of these reveals a previously unreported coordination for the magnesium ion involving four water molecules and only a tenuous connection to the protein. The higher resolution structures provide unambiguous insight into the interactions at the G-interface where a structural motif based on an antiparallel beta-bridge allows for the rationalization of why some septins show nucleotide-dependent beta-strand slippage and others do not. (AU)

Processo FAPESP: 14/15546-1 - Septinas: estudos comparativos visando correlacionar estrutura e função
Beneficiário:Richard Charles Garratt
Linha de fomento: Auxílio à Pesquisa - Temático