A Comparative Study on Chemical Composition, Antileishmanial and Cytotoxic Activities of the Essential Oils from Leaves of Guarea macrophylla (Meliaceae) from Two Different Regions of São Paulo State, Brazil, Using Multivariate Statistical Analysis

Autor(es)

Oliveira, Emerson A. ^[1] ; Martins, Euder G. A. ^[2] ; Soares, Marisi G. ^[3] ; Chagas-Paula, Daniela A. ^[3] ; Passero, Luiz F. D. ^{[4, 5]} ; Sartorelli, Patricia ^[1] ; Baldim, Joao L. ^[6] ; Lago, Joao Henrique G. ^[6]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema, SP - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, BR-05508090 Sao Paulo, SP - Brazil

^[3] Univ Fed Alfenas, Inst Quim, BR-37130001 Alfenas, MG - Brazil

^[4] Univ Estadual Paulista, Inst Biociencias, BR-11330900 Sao Vicente, SP - Brazil

^[5] Univ Estadual Paulista, Inst Estudos Avancados Mar, BR-11330900 Sao Vicente, SP - Brazil

^[6] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 30, n. 7, p. 1395-1405, JUL 2019.
• Citações Web of Science: 0

Resumo

Meliaceae representatives are economically important in several aspects including the production of highly prized woods (mahogany, cedar, etc.), constituents for cosmetics, and insecticides. The present study aimed to verify the chemical composition as well as leishmanicidal and cytotoxic potential of essential oils from leaves of two different populations of Guarea macrophylla collected at cities of São Paulo (population I) and Cubatão (population II), São Paulo State, Brazil. Chemically, the oils showed the predominance of sesquiterpenes: cis-β-guaiene, bicyclogermacrene, viridiflorol, and isolongifolan-7α-ol from population I and α-copaene, E-caryophyllene, cis-β-guaiene, and γ-amorphene from population II. In vitro antileishmanial activity against promastigote forms of Leishmania (L.) amazonensis of essential oils was evaluated and displayed 50% effective concentration (EC50) values ranging from 11.8 to 20.5 µg mL-1. Furthermore, toxicity against peritoneal macrophages of BALB/c mice was observed, with 50% cytotoxic concentration (CC50) ranging from 17.7 to > 100 µg mL-1. Multivariate statistical analysis revealed the influence of each constituent of the oils against L. amazonensis being 1,10-di-epi-cubenol, α-amorphene, E-caryophyllene, isopimara-7,15-diene, and β-elemene associated with the antileishmanial potential. (AU)

• Processo FAPESP: 16/00468-0 - Uso do reposicionamento de fármacos e da bioprospecção de produtos naturais para caracterizar compostos com ação leishmanicida in vitro e in vivo
• Beneficiário: Luiz Felipe Domingues Passero
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/07885-1 - Biomoléculas oriundas de espécies vegetais de áreas remanescentes da Mata Atlântica e do Cerrado para tratamento de doenças tropicais negligenciadas - aspectos químicos e farmacológicos
• Beneficiário: João Henrique Ghilardi Lago
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 16/19269-8 - Indução metabólica guiada por análises genômicas em Burkholderia thailandensis para a biossíntese de produtos naturais com atividade antimicrobiana
• Beneficiário: Joao Luiz Baldim Zanin
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado