Arthropod-bacteria interactions influence assembly of aquatic host microbiome and pathogen defense

Autor(es):Mostrar menos -

Greenspan, Sasha E. ^[1] ; Lyra, Mariana L. ^{[2, 3]} ; Migliorini, Gustavo H. ^[4] ; Kersch-Becker, Monica F. ^[1] ; Bletz, Molly C. ^[5] ; Lisboa, Cybele Sabino ^[6] ; Pontes, Mariana R. ^{[7, 8]} ; Ribeiro, Luisa P. ^{[7, 8]} ; Neely, Wesley J. ^[1] ; Rezende, Felipe ^[7] ; Romero, Gustavo Q. ^[9] ; Woodhams, Douglas C. ^[5] ; Haddad, Celio F. B. ^{[2, 3]} ; Toledo, Luis Felipe ^[8] ; Becker, C. Guilherme ^[1]

Afiliação do(s) autor(es)

^[1] Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35487 - USA

^[2] Univ Estadual Paulista, Dept Zool, BR-13506900 Rio Claro, SP - Brazil

^[3] Univ Estadual Paulista, Aquaculture Ctr CAUNESP, BR-13506900 Rio Claro, SP - Brazil

^[4] Univ Estadual Paulista, Programa Posgrad Biol Anim, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[5] Univ Massachusetts, Dept Biol, Boston, MA 02125 - USA

^[6] Fundacao Parque Zool Sao Paulo, BR-04301905 Sao Paulo, SP - Brazil

^[7] Univ Estadual Campinas, Inst Biol, Programa Posgrad Ecol, BR-13083862 Campinas, SP - Brazil

^[8] Univ Estadual Campinas, Dept Biol Anim, Lab Hist Nat Anfibios Brasileiros LaHNAB, BR-13083862 Campinas, SP - Brazil

^[9] Univ Estadual Campinas, Dept Biol Anim, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES; v. 286, n. 1905 JUN 2019.
• Citações Web of Science: 1

Resumo

The host-associated microbiome is vital to host immunity and pathogen defense. In aquatic ecosystems, organisms may interact with environmental bacteria to influence the pool of potential symbionts, but the effects of these interactions on host microbiome assembly and pathogen resistance are unresolved. We used replicated bromeliad microecosystems to test for indirect effects of arthropod-bacteria interactions on host microbiome assembly and pathogen burden, using tadpoles and the fungal amphibian pathogen Batrachochytrium dendrobatidis as a model host-pathogen system. Arthropods influenced host microbiome assembly by altering the pool of environmental bacteria, with arthropod-bacteria interactions specifically reducing host colonization by transient bacteria and promoting antimicrobial components of aquatic bacterial communities. Arthropods also reduced fungal zoospores in the environment, but fungal infection burdens in tadpoles corresponded most closely with arthropod-mediated patterns in microbiome assembly. This result indicates that the cascading effects of arthropods on the maintenance of a protective host microbiome may be more strongly linked to host health than negative effects of arthropods on pools of pathogenic zoospores. Our work reveals tight links between healthy ecosystem dynamics and the functioning of host microbiomes, suggesting that ecosystem disturbances such as loss of arthropods may have downstream effects on host-associated microbial pathogen defenses and host fitness. (AU)

• Processo FAPESP: 14/23388-7 - Comunicação e sistemas sensoriais em anuros da Mata Atlântica
• Beneficiário: Luis Felipe de Toledo Ramos Pereira
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/26162-8 - Diversidade e conservação dos anfíbios brasileiros
• Beneficiário: Mariana Lúcio Lyra
• Linha de fomento: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 16/03344-0 - O quitrídio nos ranários do Estado de São Paulo e suas implicações para conservação da fauna nativa
• Beneficiário: Luisa de Pontes Ribeiro
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/50741-7 - Diversidade e conservação dos anfíbios brasileiros
• Beneficiário: Célio Fernando Baptista Haddad
• Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 14/50342-8 - Anfíbios do mosaico de áreas protegidas do Lagamar: diversidade, conservação e perspectivas
• Beneficiário: Célio Fernando Baptista Haddad
• Linha de fomento: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE