Effect of CPoint, EndoSequence BC, and Gutta-percha Points on Viability and Gene Expression of Periodontal Ligament Fibroblasts

Autor(es)

Meneses, Claudia Bosio ^[1] ; Gambini, Alessandra Fonseca ^[1] ; Olivi, Lucas Tofanello ^[1] ; Dos Santos, Marcelo ^[1] ; Sipert, Carla Renata ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Restorat Dent, Sch Dent, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN ENDODONTIC JOURNAL; v. 4, n. 2, p. 57-61, 2019.
• Citações Web of Science: 0

Resumo

Objective:This study aimed to investigate the cytotoxic and biomodulatory potential of conventional guttapercha (CGP) points, gutta-percha points containing bioceramics (BC), and CPoint polymer (CP) points on periodontal ligament (PDL) cells in vitro. Methods: PDL fibroblasts were cultured and stimulated with extracts of CGP, BC, and CP in serial dilutions to evaluate cell viability using MTT assay. Next, the 1:5 dilution was used to stimulate the cells for 72 h to assess the gene expression of type I collagen (COL-1) and cement protein 1 (CEMP-1), by reverse transcription followed by quantitative PCR. Data were statistically analyzed using one-way analysis of variance (ANOVA) (P<0.05). Results: Pure extracts of CGP and CP were found to be cytotoxic for PDL (P<0.01). Once diluted to 1:5, only CP showed cytotoxicity. BC did not affect cell viability in any extract sample. No extract significantly altered the gene expression of COL-1. For CEMP-1, a significant increase in gene expression was observed only for CGP (P<0.05). Conclusion: CP was found to be more cytotoxic than CGP, while BC demonstrated no cytotoxicity. The tested cones did not affect COL-1 gene expression, while CGP upregulated CEMP-1. Our results suggest that obturation point components may affect the biological responses of PDL fibroblasts. (AU)

• Processo FAPESP: 17/01737-8 - Papel de endocanabinóides na modulação de células de papila apical humana in vitro
• Beneficiário: Claudia Caroline Bosio Meneses
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/13944-5 - Papel de mediadores inflamatórios na modulação de células de papila apical humana in vitro
• Beneficiário: Carla Renata Sipert
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/00643-0 - Efeito da guta-percha sobre a biomodulação in vitro de fibroblastos do ligamento periodontal
• Beneficiário: Lucas Tofanello Olivi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica