| Full text | |
| Author(s): |
Meneses, Claudia Bosio
[1]
;
Gambini, Alessandra Fonseca
[1]
;
Olivi, Lucas Tofanello
[1]
;
Dos Santos, Marcelo
[1]
;
Sipert, Carla Renata
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Dept Restorat Dent, Sch Dent, Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | EUROPEAN ENDODONTIC JOURNAL; v. 4, n. 2, p. 57-61, 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Objective:This study aimed to investigate the cytotoxic and biomodulatory potential of conventional guttapercha (CGP) points, gutta-percha points containing bioceramics (BC), and CPoint polymer (CP) points on periodontal ligament (PDL) cells in vitro. Methods: PDL fibroblasts were cultured and stimulated with extracts of CGP, BC, and CP in serial dilutions to evaluate cell viability using MTT assay. Next, the 1:5 dilution was used to stimulate the cells for 72 h to assess the gene expression of type I collagen (COL-1) and cement protein 1 (CEMP-1), by reverse transcription followed by quantitative PCR. Data were statistically analyzed using one-way analysis of variance (ANOVA) (P<0.05). Results: Pure extracts of CGP and CP were found to be cytotoxic for PDL (P<0.01). Once diluted to 1:5, only CP showed cytotoxicity. BC did not affect cell viability in any extract sample. No extract significantly altered the gene expression of COL-1. For CEMP-1, a significant increase in gene expression was observed only for CGP (P<0.05). Conclusion: CP was found to be more cytotoxic than CGP, while BC demonstrated no cytotoxicity. The tested cones did not affect COL-1 gene expression, while CGP upregulated CEMP-1. Our results suggest that obturation point components may affect the biological responses of PDL fibroblasts. (AU) | |
| FAPESP's process: | 17/01737-8 - Role of endocanabinoids on the modulation of human apical papilla cells in vitro |
| Grantee: | Claudia Caroline Bosio Meneses |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 16/13944-5 - Role of inflammatory mediators on the modulation of Cells from Apical Papilla: in vitro study |
| Grantee: | Carla Renata Sipert |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/00643-0 - Effect of gutta-percha on in vitro biomodulation of periodontal ligament fibroblasts |
| Grantee: | Lucas Tofanello Olivi |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |