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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of CPoint, EndoSequence BC, and Gutta-percha Points on Viability and Gene Expression of Periodontal Ligament Fibroblasts

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Author(s):
Meneses, Claudia Bosio [1] ; Gambini, Alessandra Fonseca [1] ; Olivi, Lucas Tofanello [1] ; Dos Santos, Marcelo [1] ; Sipert, Carla Renata [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Dept Restorat Dent, Sch Dent, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: EUROPEAN ENDODONTIC JOURNAL; v. 4, n. 2, p. 57-61, 2019.
Web of Science Citations: 0
Abstract

Objective:This study aimed to investigate the cytotoxic and biomodulatory potential of conventional guttapercha (CGP) points, gutta-percha points containing bioceramics (BC), and CPoint polymer (CP) points on periodontal ligament (PDL) cells in vitro. Methods: PDL fibroblasts were cultured and stimulated with extracts of CGP, BC, and CP in serial dilutions to evaluate cell viability using MTT assay. Next, the 1:5 dilution was used to stimulate the cells for 72 h to assess the gene expression of type I collagen (COL-1) and cement protein 1 (CEMP-1), by reverse transcription followed by quantitative PCR. Data were statistically analyzed using one-way analysis of variance (ANOVA) (P<0.05). Results: Pure extracts of CGP and CP were found to be cytotoxic for PDL (P<0.01). Once diluted to 1:5, only CP showed cytotoxicity. BC did not affect cell viability in any extract sample. No extract significantly altered the gene expression of COL-1. For CEMP-1, a significant increase in gene expression was observed only for CGP (P<0.05). Conclusion: CP was found to be more cytotoxic than CGP, while BC demonstrated no cytotoxicity. The tested cones did not affect COL-1 gene expression, while CGP upregulated CEMP-1. Our results suggest that obturation point components may affect the biological responses of PDL fibroblasts. (AU)

FAPESP's process: 17/01737-8 - Role of endocanabinoids on the modulation of human apical papilla cells in vitro
Grantee:Claudia Caroline Bosio Meneses
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 16/13944-5 - Role of inflammatory mediators on the modulation of Cells from Apical Papilla: in vitro study
Grantee:Carla Renata Sipert
Support Opportunities: Regular Research Grants
FAPESP's process: 17/00643-0 - Effect of gutta-percha on in vitro biomodulation of periodontal ligament fibroblasts
Grantee:Lucas Tofanello Olivi
Support Opportunities: Scholarships in Brazil - Scientific Initiation