Role of inflammatory mediators on the modulation of Cells from Apical Papilla: in vitro study

• Grant number: 16/13944-5
• Support Opportunities: Regular Research Grants
• Duration: October 01, 2016 - September 30, 2018
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Carla Renata Sipert
• Grantee: Carla Renata Sipert
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The endodontic treatment of immature teeth is considered one of the greatest challenges of contemporary Endodontics. Despite the recent advances in this area of Dentistry, the molecular mechanisms involved with interruption of root formation resulting from dental caries or trauma are still unknown. Therefore, the aim of this study is to elucidate the role of inflammatory mediators in functional alterations of apical papilla cells (CPA). In vitro cultures of CPA will be established from third molars with incomplete root formation. The cells will be subjected to stimulum with increasing concentrations of lipopolysaccharide (LPS) for cellular viability assay. Then, CPA will be challenged with LPS at proliferation (MP) or differentiation medium (MD) for detection of prostaglandin E2 (PGE2), endocanabinoids or angiotensin II (Ang II) production and their respective receptors. Based on these results, the experimental conditions and receptors will be determined for blockade at the following assays. The role of PGE2 (EP1-4), endocanabinoid (CB1 and CB2) and angiotensin receptor 1 (AT1) receptors at the CPA function will be investigated by means of pharmacological blockade. The context of cellular differentiation and the envolvment of these inflammatory mediators at the inflammatory bone resorption mechanisms will be evaluated. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARLUS DA SILVA PEDROSA; TOMAZ ALVES; FERNANDO NEVES NOGUEIRA; MARINELLA HOLZHAUSEN; CARLA RENATA SIPERT. Cytotoxicity and cytokine production by calcium silicate-based materials on periodontal ligament stem cells. Brazilian Dental Journal, v. 32, n. 3, p. 65-74, 2021-11-08. (17/23158-0, 18/13818-5, 16/13944-5)

PEDROSA, MARLUS DA SILVA; ALVES, TOMAZ; RAHHAL, JULIANA GARUBA; NOGUEIRA, FERNANDO NEVES; SIPERT, CARLA RENATA. ytotoxicity of Reparative Endodontic Cements on Human Periodontal Ligament Stem Cell. PESQUISA BRASILEIRA EM ODONTOPEDIATRIA E CLINICA INTEGRADA, v. 22, 2022. (17/23158-0, 16/13944-5)

MENESES, CLAUDIA BOSIO; GAMBINI, ALESSANDRA FONSECA; OLIVI, LUCAS TOFANELLO; DOS SANTOS, MARCELO; SIPERT, CARLA RENATA. Effect of CPoint, EndoSequence BC, and Gutta-percha Points on Viability and Gene Expression of Periodontal Ligament Fibroblasts. EUROPEAN ENDODONTIC JOURNAL, v. 4, n. 2, p. 57-61, 2019. (17/01737-8, 16/13944-5, 17/00643-0)

SIPERT, CARLA RENATA; OLIVEIRA, ALINE PEREIRA; CALDEIRA, CELSO LUIZ. Cytotoxicity of intracanal dressings on apical papilla cells differ upon activation with E. faecalis LTA. Journal of Applied Oral Science, v. 27, 2019. (16/13944-5)

RAHHAL, JULIANA GARUBA; ROVAI, EMANUEL DA SILVA; HOLZHAUSEN, MARINELLA; CALDEIRA, CELSO LUIZ; DOS SANTOS, CARLOS FERREIRA; SIPERT, CARLA RENATA. Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells. Journal of Applied Oral Science, v. 27, 2019. (15/09750-8, 15/03965-2, 16/13944-5, 15/11587-8)

MARLUS DA SILVA PEDROSA; TOMAZ ALVES; JULIANA GARUBA RAHHAL; FERNANDO NEVES NOGUEIRA; CARLA RENATA SIPERT. Cytotoxicity of Reparative Endodontic Cements on Human Periodontal Ligament Stem Cells. Pesquisa Brasileira em Odontopediatria e Clínica Integrada, v. 22, 2022-03-07. (17/23158-0, 16/13944-5)

BOSIO MENESES, CLAUDIA CAROLINE; PIZZATTO, LAIS NICOLAY; FARES ANDRADE, FERNANDA FACHINI; SIPERT, CARLA RENATA. Prostaglandin E-2 Affects Interleukin 6 and Monocyte Chemoattractant Protein 1/CCL2 Production by Cultured Stem Cells of Apical Papilla. JOURNAL OF ENDODONTICS, v. 46, n. 3, p. 413-418, MAR 2020. (16/13944-5, 16/16473-3, 17/01737-8)

MARLUS DA SILVA PEDROSA; FERNANDO NEVES NOGUEIRA; CARLA RENATA SIPERT. Calcium silicate-based cements affect the cell viability and the release of TGF-β1 from apical papilla cells. Brazilian Dental Journal, v. 32, n. 6, p. 1-7, 2022-01-05. (16/13944-5)

PIZZATTO, LAIS NICOLAY; MENESES, CLAUDIA C. B.; DINIZ, ELISA A.; DIONISIO, THIAGO J.; SANTOS, CARLOS FERREIRA; SIPERT, CARLA R.. Angiotensin II Regulates Proliferation and Function of Stem Cells of Apical Papilla. JOURNAL OF ENDODONTICS, v. 46, n. 6, p. 810-817, JUN 2020. (17/01737-8, 16/16473-3, 16/13944-5, 15/03965-2)

BOSIO MENESES, CLAUDIA CAROLINE; OLIVI, LUCAS TOFANELLO; CARVALHO, CECI NUNES; GAVINI, GIULIO; SIPERT, CARLA RENATA. Cytotoxic Effect of Niobium Phosphate Glass-based Gutta-Percha Points on Periodontal Ligament Fibroblasts In Vitro. JOURNAL OF ENDODONTICS, v. 46, n. 9, p. 5-pg., 2020-09-01. (12/18220-4, 17/00643-0, 17/01737-8, 16/13944-5)

MENESES, CLAUDIA CAROLINE BOSIO; PIZZATTO, LAIS N.; SIPERT, CARLA R.; DIOGENES, ANIBAL. Endocannabinoids Regulate Stem Cells of the Apical Papilla via a Cannabinoid Receptor and TRPV1-Independent Mechanism. JOURNAL OF ENDODONTICS, v. 47, n. 10, p. 1617-1624, OCT 2021. (16/16473-3, 16/13944-5, 18/21869-9, 17/01737-8)