Role of endocanabinoids on the modulation of human apical papilla cells in vitro

• Grant number: 17/01737-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): May 01, 2017
• Effective date (End): August 09, 2020
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Carla Renata Sipert
• Grantee: Claudia Caroline Bosio Meneses
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 18/21869-9 - The role of endocannabinoids in the modulation of apical papilla cells: an in vitro study, BE.EP.DR

Abstract

The cells of apical papila (CPA) have direct influence in the root formation during dental development, acting as a source of dentin-synthesizing cells. If a pulpal pathological process is installed before the end of rhizogeneses, there is the possibility of pulpal necrosis and interruption of root formation. Although this cause/effect relationship is known, the molecular mechanisms involved in the process of rhizogeneses interruption and its relationship with CPA have not been clarified. Among the mediators potencially involved in this process, the endocannabinoids (eCBs) can be mentioned, which act in the modulation of several cellular activities. The aim of this study is to elucidate the role of eCBs in the molecular mechanisms involved with phenotypic and functional alterations of CPA in vitro. CPA cultures will be established and characterized from healthy immature third molars. Cells will be subjected to cellular viability assay through exposure to increasing concentrations of lipopolysaccharide (LPS). CPA will be challenged with LPS at proliferation (MP) and differentiation medium (MD) to detect the production of eCBs enzymatic system and expression of their receptors (CB1 and CB2). The influence of these receptors on the cellular modulation will be analyzed by means of pharmocological blockade. The role of CB1 and CB2 in cell differentiation and modulation of inflammatory bone resorption induced by LPS will be evaluated. Statistical analysis will be performed using one-way analysis of variance (ANOVA) followed by Tukey's post-test or Student's test. Significant values will be set at p<0,05. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENESES, CLAUDIA BOSIO; GAMBINI, ALESSANDRA FONSECA; OLIVI, LUCAS TOFANELLO; DOS SANTOS, MARCELO; SIPERT, CARLA RENATA. Effect of CPoint, EndoSequence BC, and Gutta-percha Points on Viability and Gene Expression of Periodontal Ligament Fibroblasts. EUROPEAN ENDODONTIC JOURNAL, v. 4, n. 2, p. 57-61, 2019. (17/01737-8, 16/13944-5, 17/00643-0)

BOSIO MENESES, CLAUDIA CAROLINE; PIZZATTO, LAIS NICOLAY; FARES ANDRADE, FERNANDA FACHINI; SIPERT, CARLA RENATA. Prostaglandin E-2 Affects Interleukin 6 and Monocyte Chemoattractant Protein 1/CCL2 Production by Cultured Stem Cells of Apical Papilla. JOURNAL OF ENDODONTICS, v. 46, n. 3, p. 413-418, MAR 2020. (16/13944-5, 16/16473-3, 17/01737-8)

PIZZATTO, LAIS NICOLAY; MENESES, CLAUDIA C. B.; DINIZ, ELISA A.; DIONISIO, THIAGO J.; SANTOS, CARLOS FERREIRA; SIPERT, CARLA R.. Angiotensin II Regulates Proliferation and Function of Stem Cells of Apical Papilla. JOURNAL OF ENDODONTICS, v. 46, n. 6, p. 810-817, JUN 2020. (17/01737-8, 16/16473-3, 16/13944-5, 15/03965-2)

BOSIO MENESES, CLAUDIA CAROLINE; OLIVI, LUCAS TOFANELLO; CARVALHO, CECI NUNES; GAVINI, GIULIO; SIPERT, CARLA RENATA. Cytotoxic Effect of Niobium Phosphate Glass-based Gutta-Percha Points on Periodontal Ligament Fibroblasts In Vitro. JOURNAL OF ENDODONTICS, v. 46, n. 9, p. 5-pg., 2020-09-01. (12/18220-4, 17/00643-0, 17/01737-8, 16/13944-5)

MENESES, CLAUDIA CAROLINE BOSIO; PIZZATTO, LAIS N.; SIPERT, CARLA R.; DIOGENES, ANIBAL. Endocannabinoids Regulate Stem Cells of the Apical Papilla via a Cannabinoid Receptor and TRPV1-Independent Mechanism. JOURNAL OF ENDODONTICS, v. 47, n. 10, p. 1617-1624, OCT 2021. (16/16473-3, 16/13944-5, 18/21869-9, 17/01737-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MENESES, Claudia Caroline Bosio. The role of Endocannabinoids in the modulation of stem cells of the Apical Papilla in vitro. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) São Paulo.