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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prostaglandin E-2 Affects Interleukin 6 and Monocyte Chemoattractant Protein 1/CCL2 Production by Cultured Stem Cells of Apical Papilla

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Author(s):
Bosio Meneses, Claudia Caroline [1] ; Pizzatto, Lais Nicolay [1] ; Fares Andrade, Fernanda Fachini [1] ; Sipert, Carla Renata [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Av Prof Lineu Prestes, 2227, Cidade Univ, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF ENDODONTICS; v. 46, n. 3, p. 413-418, MAR 2020.
Web of Science Citations: 1
Abstract

Introduction: Root canal treatment of immature necrotic teeth is a major challenge in current endodontics. The effect of inflammatory mediators, such as prostaglandin, on the modulation of stem cells of the apical papilla (SOAP) is not completely understood. The aim of this study was to investigate the role of prostaglandin E-2 (PGE(2)) on SOAP activation by Escherichia coli lipopolysaccharide (LPS) in vitro. Methods: SOAP cultures were established and characterized. Increasing concentrations of lipopolysaccharide (0.1-10 mu g/mL) were used to investigate cyclooxygenase-2 (COX-2/PTGS2) and PGE(2) receptors (EP1-4) gene expression. Then, SOAP were treated with a COX-2 inhibitor (indomethacin) before treatment with different concentrations of LPS. The levels of the chemokine CCL2/monocyte chemo-attractant protein 1 and interleukin (IL)-6 were detected in cell supernatants (24 hours) by enzyme-linked immunosorbent assay. Data analysis was performed using analysis of variance followed by the Tukey post test. Results: The expression of COX-2 was up-regulated in the group treated with LPS at 1 mu g/mL compared with that in the control group. EP1-4 were detected in all experimental conditions at similar levels. SOAP treated with indomethacin presented a down-regulation in the production of LPS-induced CCL2 and the secretion of IL-6. Conclusions: SOAP showed increased COX-2 (PTGS2) gene expression induced by LPS and a PGE2-dependent production of IL-6 and CCL2. (AU)

FAPESP's process: 16/13944-5 - Role of inflammatory mediators on the modulation of Cells from Apical Papilla: in vitro study
Grantee:Carla Renata Sipert
Support Opportunities: Regular Research Grants
FAPESP's process: 16/16473-3 - Study of the role of angiotensin II on in vitro differentiation of human apical papilla cells
Grantee:Laís Nicolay Pizzatto
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 17/01737-8 - Role of endocanabinoids on the modulation of human apical papilla cells in vitro
Grantee:Claudia Caroline Bosio Meneses
Support Opportunities: Scholarships in Brazil - Doctorate