Genomic Features of High-Priority Salmonella enterica Serovars Circulating in the Food Production Chain, Brazil, 2000-2016

Autor(es)

Monte, Daniel F. . ^{[1, 2]} ; Lincopan, Nilton ^{[3, 4]} ; Berman, Hanna ^[1] ; Cerdeira, Louise ^[4] ; Keelara, Shivaramu ^[1] ; Thakur, Siddhartha ^[1] ; Fedorka-Cray, Paula J. ^[1] ; Landgraf, Mariza ^[2]

Afiliação do(s) autor(es)

^[1] North Carolina State Univ, Coll Vet Med, Dept Populat Hlth & Pathobiol, Raleigh, NC 27695 - USA

^[2] Univ Sao Paulo, Food Res Ctr, Fac Pharmaceut Sci, Dept Food & Expt Nutr, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, JUL 30 2019.
• Citações Web of Science: 0

Resumo

Multidrug-resistant (MDR) Salmonella enterica has been deemed a high-priority pathogen by the World Health Organization. Two hundred and sixty-four Salmonella enterica isolates recovered over a 16-year period (2000 to 2016) from the poultry and swine production chains, in Brazil, were investigated by whole-genome sequencing (WGS). Most international lineages belonging to 28 serovars, including, S. enterica serovars S. Schwarzengrund ST96, S. Typhimurium ST19, S. Minnesota ST548, S. Infantis ST32, S. Heidelberg ST15, S. Newport ST45, S. Brandenburg ST65 and S. Kentucky ST198 displayed MDR and virulent genetic backgrounds. In this regard, resistome analysis revealed presence of qnrE1 (identified for the first time in S. Typhimurium from food chain), qnrB19, qnrS1, bla(CTX-M-8), bla(CTX-M-2) and bla(CMY-2) genes, as well as gyrA mutations; whereas ColpVC, IncHI2A, IncHI2, IncFIA, Incl1, IncA/C2, IncR, IncX1 and po111 plasmids were detected. In addition, phylogenetic analysis revealed multiple independent lineages such as S. enterica serovars S. Infantis, S. Schwarzengrund, S. Minnesota, S. Kentucky and S. Brandenburg. In brief, ocurrence and persistence of international lineages of S. enterica serovars in food production chain is supported by conserved genomes and wide virulome and resistome. (AU)

• Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
• Beneficiário: Bernadette Dora Gombossy de Melo Franco
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 17/15967-5 - Abordagem de sequenciamento do genoma completo para estudar Salmonella enterica multirresistentes isoladas de abatedouros do Brasil
• Beneficiário: Daniel Farias Marinho Do Monte
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 16/03044-7 - Diversidade genética, genes de virulência e resistência antimicrobiana de isolados de Salmonella spp. provenientes de abatedouros de frango
• Beneficiário: Daniel Farias Marinho Do Monte
• Modalidade de apoio: Bolsas no Brasil - Doutorado