Microbial Community Profiling in Intensive Care Units Expose Limitations in Current Sanitary Standards

Autor(es)

Ribeiro, Lucas Ferreira ^[1] ; Lopes, Erica M. ^[2] ; Kishi, Luciano T. ^[3] ; Costa Ribeiro, Liliane Fraga ^[4] ; Menegueti, Mayra Goncalves ^[5] ; Gaspar, Gilberto Gambero ^[5] ; Silva-Rocha, Rafael ^[2] ; Guazzaroni, Maria-Eugenia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FFCLRP, Dept Biol, Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, FMRP, Dept Cellular & Mol Biol, Ribeirao Preto - Brazil

^[3] Natl Lab Sci Comp, Petropolis - Brazil

^[4] Univ Sao Paulo, FMRP, Dept Biochem & Immunol, Ribeirao Preto - Brazil

^[5] Univ Sao Paulo, Med Sch Clin Hosp, Infect Control Serv, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PUBLIC HEALTH; v. 7, AUG 28 2019.
• Citações Web of Science: 0

Resumo

Hospital-associated infections (HAIs) are a leading cause of morbidity and mortality in intensive care units (ICUs) and neonatal intensive care units (NICUs). Organisms causing these infections are often present on surfaces around the patient. Given that microbiota may vary across different ICUs, the HAI-related microbial signatures within these units remain underexplored. In this study, we use deep-sequencing analyses to explore and compare the structure of bacterial communities at inanimate surfaces of the ICU and NICU wards of The Medical School Clinics Hospital (Brazil). The data revealed that NICU presents higher biodiversity than ICU and surfaces closest to the patient showed a peculiar microbiota, distinguishing one unit from the other. Several facultative anaerobes or obligate anaerobes HAI-related genera were classified as biomarkers for the NICU, whereas Pseudomonas was the main biomarker for ICU. Correlation analyses revealed a distinct pattern of microbe-microbe interactions for each unit, including bacteria able to form multi-genera biofilms. Furthermore, we evaluated the effect of concurrent cleaning over the ICU bacterial community. The results showed that, although some bacterial populations decreased after cleaning, various HAI-related genera were quite stable following sanitization, suggesting being well-adapted to the ICU environment. Overall, these results enabled identification of discrete ICU and NICU reservoirs of potentially pathogenic bacteria and provided evidence for the presence of a set of biomarkers genera that distinguish these units. Moreover, the study exposed the inconsistencies of the routine cleaning to minimize HAI-related genera contamination. (AU)

• Processo FAPESP: 15/04309-1 - Novas abordagens para melhorar a prospecção funcional de biocatalizadores em bibliotecas metagenômicas
• Beneficiário: María Eugenia Guazzaroni
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 16/20358-5 - Análise sistêmica do papel da proteína quinase A (PKA) no fungo Trichoderma reesei através de fosfoproteômica
• Beneficiário: Liliane Fraga Costa Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/18827-7 - Integrando engenharia de proteínas e biologia sintética para desenvolver novos sistemas de high-throughput selection
• Beneficiário: Lucas Ferreira Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado