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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Microbial Community Profiling in Intensive Care Units Expose Limitations in Current Sanitary Standards

Full text
Author(s):
Ribeiro, Lucas Ferreira [1] ; Lopes, Erica M. [2] ; Kishi, Luciano T. [3] ; Costa Ribeiro, Liliane Fraga [4] ; Menegueti, Mayra Goncalves [5] ; Gaspar, Gilberto Gambero [5] ; Silva-Rocha, Rafael [2] ; Guazzaroni, Maria-Eugenia [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, FFCLRP, Dept Biol, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, FMRP, Dept Cellular & Mol Biol, Ribeirao Preto - Brazil
[3] Natl Lab Sci Comp, Petropolis - Brazil
[4] Univ Sao Paulo, FMRP, Dept Biochem & Immunol, Ribeirao Preto - Brazil
[5] Univ Sao Paulo, Med Sch Clin Hosp, Infect Control Serv, Ribeirao Preto - Brazil
Total Affiliations: 5
Document type: Journal article
Source: FRONTIERS IN PUBLIC HEALTH; v. 7, AUG 28 2019.
Web of Science Citations: 0
Abstract

Hospital-associated infections (HAIs) are a leading cause of morbidity and mortality in intensive care units (ICUs) and neonatal intensive care units (NICUs). Organisms causing these infections are often present on surfaces around the patient. Given that microbiota may vary across different ICUs, the HAI-related microbial signatures within these units remain underexplored. In this study, we use deep-sequencing analyses to explore and compare the structure of bacterial communities at inanimate surfaces of the ICU and NICU wards of The Medical School Clinics Hospital (Brazil). The data revealed that NICU presents higher biodiversity than ICU and surfaces closest to the patient showed a peculiar microbiota, distinguishing one unit from the other. Several facultative anaerobes or obligate anaerobes HAI-related genera were classified as biomarkers for the NICU, whereas Pseudomonas was the main biomarker for ICU. Correlation analyses revealed a distinct pattern of microbe-microbe interactions for each unit, including bacteria able to form multi-genera biofilms. Furthermore, we evaluated the effect of concurrent cleaning over the ICU bacterial community. The results showed that, although some bacterial populations decreased after cleaning, various HAI-related genera were quite stable following sanitization, suggesting being well-adapted to the ICU environment. Overall, these results enabled identification of discrete ICU and NICU reservoirs of potentially pathogenic bacteria and provided evidence for the presence of a set of biomarkers genera that distinguish these units. Moreover, the study exposed the inconsistencies of the routine cleaning to minimize HAI-related genera contamination. (AU)

FAPESP's process: 15/04309-1 - Novel approaches to improve functional screening of biocatalysts in metagenomic libraries
Grantee:María Eugenia Guazzaroni
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 16/20358-5 - Systems biology analysis of the role of protein kinase A (PKA), in the fungus Trichoderma reesei by phosphoproteomic
Grantee:Liliane Fraga Costa Ribeiro
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/18827-7 - Integrating protein engineering and synthetic biology to develop new high-throughput selection systems
Grantee:Lucas Ferreira Ribeiro
Support Opportunities: Scholarships in Brazil - Post-Doctoral