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Estimative of probability of infection associate of biosolids utilization as soil amendment using quantitative microbial risk assessment (QMRA)

Grant number: 10/05664-6
Support Opportunities:Regular Research Grants
Duration: September 01, 2010 - August 31, 2012
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Maria Tereza Pepe Razzolini
Grantee:Maria Tereza Pepe Razzolini
Host Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Biosolids have been used in agricultural soil and crops, but this practice brings up some concerns regarding acute and chronicle effects to human and environmental health due to pathogens presence. Quantitative Microbial Risk Assessment (QMRA) is a useful tool to estimate the risk of infection to an exposed population considering a specific scenario. Objective: The aim of this study is to evaluate biosolid sanitary quality according to Resolução CONAMA 375/2006 and estimate the probability of infection by Salmonella e Ascaris lumbricoides related to exposure to these etiologic agents when biosolids are used as soil amendment. Methods: Samples will be collected from six sewage treatment units (STU), located in São Paulo state, spanning 12 months being in each STU samplings will occur every second month comprising 36 samples. Sampling will carry out according to analytical method ABNT/NBR10.0007/2004. Total solids' parameter will be determined following Standards Methods (21st ed. 2005), SM 2540. The enumeration of thermotolerants coliforms and E. coli will be carried out by chromogenic substrate using kit Colilert (Iddex) according to Standards Methods (21st ed. 2005), SM 9223. Salmonella quantification will be conduct using multiple-tube technique according to 1682/EPA Method (2006), Salmonella in sewage sludge (Biosolids) by modified semisolid Rappaport-Vassiliadis (MSRV) medium. The quantification and viability of helminth eggs and Ascaris lumbricoides will be carried out according to Appendix I - Test method for detecting, enumerating, and determining the viability of Ascaris ova in sludge - USEPA 2003 - Control of pathogens and vector attraction in sewage sludge. Probability of infection will be performed using exponential dose response model for helminth eggs and A.lumbricoides and ²-Poisson dose response model for Salmonella. The risk model will be subjected to a Monte Carlo uncertainty analysis to elucidate the uncertainties in the QMRA. Expected Results: Collaborating with data to support discussions about quality standards and achieve background to elaborate or review the current quality standards regarding biosolids and their application as soil amendment as well as estimate the probability of infection due to exposure to Salmonella and A. lumbricoides. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BASTOS, VERIDIANA KARMANN; CUTOLO, SILVANA AUDRA; OLIVEIRA DORIA, MARIA DO CARMO; PEPE RAZZOLINI, MARIA TEREZA. Detection and quantification of viable Ascaris sp and other helminth eggs in sewage sludge. INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH, v. 23, n. 4, p. 352-362, . (10/05664-6)
KRZYZANOWSKI, JR., FLAVIO; ZAPPELINI, LINCOHN; MARTONE-ROCHA, SOLANGE; DROPA, MILENA; MATTE, MARIA HELENA; NACACHE, FLAVIA; PEPE RAZZOLINI, MARIA TEREZA. Quantification and characterization of Salmonella spp. isolates in sewage sludge with potential usage in agriculture. BMC Microbiology, v. 14, . (10/05664-6)
KRZYZANOWSKI, JR., FLAVIO; LAURETTO, MARCELO DE SOUZA; NARDOCCI, ADELAIDE CASSIA; ZANOLI SATO, MARIA INES; PEPE RAZZOLINI, MARIA TEREZA. Assessing the probability of infection by Salmonella due to sewage sludge use in agriculture under several exposure scenarios for crops and soil ingestion. Science of The Total Environment, v. 568, p. 66-74, . (10/05664-6)

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