Green tea catechins alone or in combination alter functional parameters of human neutrophils via suppressing the activation of TLR-4/NF kappa B p65 signal pathway

Autor(es)

Marinovic, M. P. ^[1] ; Morandi, A. C. ^[1] ; Otton, R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Cruzeiro Sul, Postgrad Program Hlth Sci CBS, BR-03342000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXICOLOGY IN VITRO; v. 29, n. 7, p. 1766-1778, OCT 2015.
• Citações Web of Science: 24

Resumo

The purpose of this study was to evaluate the potential of a mixture containing the four main catechins found in green tea, as well it separately, as modulators of the functional parameters of human neutrophils. The cells were obtained from peripheral blood of healthy individuals isolated and cultured with a mix: 30 mu M of EGCG, 3 mu M of EGC, 2 mu M of ECG and 1.4 mu M of EC, as well as each one alone. We evaluated the cytotoxicity of catechins, production of several reactive oxygen species (ROS), antioxidant enzymes (SOD, CAT, GPx and GR), Nrf2, TLR4/IKK/NF kappa B, CD11b mRNA levels, intracellular calcium release, chemotactic and phagocytic capacity, myeloperoxidase (MPO), and G6PDH activities, hypochlorous acid (HOCl) and pro-inflammatory cytokines release, protein levels of TLR4, p38 MAPK, iNOS and p-65 NF kappa B. The actions of the catechins were evidenced by the reduction in inflammatory parameters, including the suppression of TLR4, NF kappa B and iNOS protein expression, decreased release of TNF-alpha, IL-1 beta and IL-6, migration capacity, MPO activity and HOCl production and the suppression of ROS, nitric oxide and peroxynitrite production, while inducing antioxidant enzyme activities and Nrf2 mRNA levels, phagocytic capacity and calcium release. Our results demonstrate that catechins present marked immunomodulatory actions, either alone or in combination. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/19216-8 - Avaliação sistemática de parâmetros oxidativos e inflamatórios em ratos obesos suplementados cronicamente com extrato de chá verde (Camellia sinensis)
• Beneficiário: Rosemari Otton
• Modalidade de apoio: Auxílio à Pesquisa - Regular