Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Pattern of sympathetic vasomotor activity in a model of hypertension induced by nitric oxide synthase blockade

Texto completo
Autor(es):
Zambrano, I, Lysien ; Pontes, Roberto B. [1] ; Garcia, Michelle L. [1] ; Nishi, Erika E. [1] ; Nogueira, Fernando N. [2] ; Higa, Elisa M. S. [3] ; Cespedes, Juliana G. [4] ; Bergamaschi, Cassia T. [1] ; Campos, Ruy R. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Zambrano, Lysien, I, Univ Fed Sao Paulo, Escola Paulista Med, Cardiovasc Div, Dept Physiol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Biomat & Biol Oral, Fac Odontol, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Escola Paulista Med, Nephrol Div, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Inst Sci & Technol, Sao Jose Dos Campos - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: PHYSIOLOGICAL REPORTS; v. 7, n. 14 JUL 2019.
Citações Web of Science: 0
Resumo

We aimed to investigate the effects of nitric oxide (NO) synthesis inhibition by NO synthase inhibitor N-nitro-L-arginine-methyl ester (L-NAME) treatment on the sympathetic vasomotor nerve activity (SNA) on two sympathetic vasomotor nerves, the renal and splanchnic. NO plasma level and systemic oxidative stress were assessed. Hypertension was induced by L-NAME (20mg/kg per day, by gavage, for seven consecutive days) in male Wistar rats. At the end of the treatment, blood pressure, heart rate, arterial baroreflex sensitivity, renal SNA (rSNA), and splanchnic SNA (sSNA) were assessed in urethane anesthetized rats. L-NAME-treated rats presented increased blood pressure (152 +/- 2mmHg, n=17) compared to the control group (101 +/- 2 mm Hg, n=15). Both rSNA (147 +/- 10, n=15 vs. 114 +/- 5 Spikes/s, n=9) and sSNA (137 +/- 13, n=14 vs. 74 +/- 13 spikes/s, n=9) were significantly increased in the L-NAME-treated compared to the control group. A differential response on baroreflex sensitivity was found, with a significant reduction for rSNA but not for sSNA arterial baroreceptor sensitivity in L-NAME-treated rats. The adjusted regression model revealed that the reduction of systemic NO levels partially explains the variation in sSNA and blood pressure, but not rSNA. Taken together, our data show that hypertension induced by NO synthase blockade is characterized by increased SNA to the rSNA and sSNA. In addition, we found that the rats that had the greatest reduction in NO levels in plasma by L-NAME were those that developed higher blood pressure levels. The reduction in the NO level partially explains the variations in sSNA but not in rSNA. (AU)

Processo FAPESP: 18/02671-3 - Ações funcionais e moleculares do nervo renal na doença renal crônica experimental
Beneficiário:Cassia Marta de Toledo Bergamaschi
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/22522-9 - Papel do nervo simpático renal nas alterações cardiovasculares e renais na hipertensão arterial renovascular
Beneficiário:Ruy Ribeiro de Campos Junior
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/23741-6 - Avaliação dos mecanismos centrais do reflexo reno-renal e o papel dos nervos aferentes renais na hipertensão renovascular
Beneficiário:Erika Emy Nishi
Linha de fomento: Bolsas no Brasil - Pós-Doutorado