| Texto completo | |
| Autor(es): |
de Moura, Nelci A.
[1]
;
Caetano, Brunno F. R.
[1]
;
Bidinotto, Lucas T.
[2, 3]
;
Rodrigues, Maria A. M.
[4]
;
Barbisan, Luis F.
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, UNESP, Botucatu, SP - Brazil
[2] Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos, SP - Brazil
[3] Dr Paulo Prata FACISB, Barretos Sch Hlth Sci, Barretos, SP - Brazil
[4] Sao Paulo State Univ, Sch Med, Dept Pathol, UNESP, Botucatu, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS; v. 846, OCT 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
Red and processed meat consumption has been strongly related to increase the risk of colorectal cancer (CRC), although its impact is largely unknown. Hemin, an iron-containing porphyrin, is acknowledged as a putative factor of red and processed meat pro-carcinogenic effects. The aim of this study was to investigate the effects of high dietary hemin on the promotion/progression stages of 1,2-dimethylhydrazine (1,2-DMH)-induced colon carcinogenesis. Twenty-four Wistar male rats were given four subcutaneous 1,2-DMH injections and received either balanced diet or balanced diet supplemented with hemin 0.5 mmol/kg for 23 weeks. Colon specimens were analyzed for aberrant crypt foci (ACF) and tumor development. Dietary hemin significantly increased ACF number and fecal water cytotoxicity/genotoxicity in Caco-2 cells when compared to 1,2-DMH control group. However, tumor incidence, multiplicity and cell proliferation did not differ between 1,2-DMH + hemin and 1,2-DMH control group. Gene expression analysis of 91 target-genes revealed that only three genes (Figf, Pik3r5 and Tgfbr2) were down-regulated in the tumors from hemin-fed rats compared to those from 1,2-DMH control group. Therefore, the findings of this study show that high hemin intake promotes mainly DNA damage and ACF development and but does not change the number nor incidence of colon tumors induced by 1,2-DMH in male rats. (AU) | |
| Processo FAPESP: | 11/23699-4 - Efeitos da ingestão de simbiótico e indol 3- carbinol sobre o processo de carcinogênese química de cólon em ratos Wistar alimentados com dieta contendo heme. |
| Beneficiário: | Nelci Antunes de Moura |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 12/12631-2 - Efeitos de simbióticos e indol 3-carbinol na expressão de genes relacionados à carcinogênese de cólon em ratos alimentados com dieta contendo heme |
| Beneficiário: | Luís Fernando Barbisan |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 13/08033-5 - Genotoxicidade da água fecal e desenvolvimento de focos de criptas aberrantes em ratos Wistar alimentados com heme, indol-3-carbinol e/ou simbiótico |
| Beneficiário: | Brunno Felipe Ramos Caetano |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |