| Full text | |
| Author(s): |
de Moura, Nelci A.
[1]
;
Caetano, Brunno F. R.
[1]
;
Bidinotto, Lucas T.
[2, 3]
;
Rodrigues, Maria A. M.
[4]
;
Barbisan, Luis F.
[1]
Total Authors: 5
|
| Affiliation: | [1] Sao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, UNESP, Botucatu, SP - Brazil
[2] Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos, SP - Brazil
[3] Dr Paulo Prata FACISB, Barretos Sch Hlth Sci, Barretos, SP - Brazil
[4] Sao Paulo State Univ, Sch Med, Dept Pathol, UNESP, Botucatu, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS; v. 846, OCT 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Red and processed meat consumption has been strongly related to increase the risk of colorectal cancer (CRC), although its impact is largely unknown. Hemin, an iron-containing porphyrin, is acknowledged as a putative factor of red and processed meat pro-carcinogenic effects. The aim of this study was to investigate the effects of high dietary hemin on the promotion/progression stages of 1,2-dimethylhydrazine (1,2-DMH)-induced colon carcinogenesis. Twenty-four Wistar male rats were given four subcutaneous 1,2-DMH injections and received either balanced diet or balanced diet supplemented with hemin 0.5 mmol/kg for 23 weeks. Colon specimens were analyzed for aberrant crypt foci (ACF) and tumor development. Dietary hemin significantly increased ACF number and fecal water cytotoxicity/genotoxicity in Caco-2 cells when compared to 1,2-DMH control group. However, tumor incidence, multiplicity and cell proliferation did not differ between 1,2-DMH + hemin and 1,2-DMH control group. Gene expression analysis of 91 target-genes revealed that only three genes (Figf, Pik3r5 and Tgfbr2) were down-regulated in the tumors from hemin-fed rats compared to those from 1,2-DMH control group. Therefore, the findings of this study show that high hemin intake promotes mainly DNA damage and ACF development and but does not change the number nor incidence of colon tumors induced by 1,2-DMH in male rats. (AU) | |
| FAPESP's process: | 11/23699-4 - Effects of symbiotic and indole-3-carbinol intake on colon carcinogenesis process in Wistar rats fed a diet containing heme. |
| Grantee: | Nelci Antunes de Moura |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/12631-2 - Effects of symbiotic and indole 3-carbinol on the gene expression involved in colon carcinogenesis in rats fed a diet containing heme |
| Grantee: | Luís Fernando Barbisan |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/08033-5 - Fecal water genotoxicity and development of aberrant crypt foci in male Wistar rats fed heme, indole-3-carbinol and/or symbiotic formulation |
| Grantee: | Brunno Felipe Ramos Caetano |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |