Production of conidia of the entomopathogenic fungus Metarhizium anisopliae ICB 425 in a tray bioreactor

Autor(es)

da Cunha, Lucas Portilho ^[1] ; Casciatori, Fernanda Perpetua ^[2] ; Lopes, Isabella de Cenco ^[1] ; Thomeo, Joao Claudio ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci Letters & Exact Sci, Food Engn & Technol Dept, Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Fed Univ Sao Carlos UFSCar, Chem Engn Dept, Rod Washington Luiz, Km 235, SP 310, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Bioprocess and Biosystems Engineering; v. 42, n. 11, p. 1757-1768, NOV 2019.
• Citações Web of Science: 0

Resumo

The use of Metarhizium anisopliae as a bioinsecticide is steeply increasing worldwide. However, to reduce the production costs, it is necessary to develop sophisticated techniques for conidia production. This work aimed to use a tray bioreactor to produce conidia of M. anisopliae ICB-425 in long rice and find the limiting bed depth in which the production is still viable. Experiments have been carried out to assess the influence of the air temperature and relative humidity on the spore concentration in order to determine the limiting temperature. Two scales of bioreactors in plastic packages have been used, containing 10 and 500 g of rice, and the results were similar. In the tray bioreactor, the bed depths of 2, 4 and 6 cm have been used, corresponding to the dry rice weights of 1, 2 and 3 kg, respectively, and the results were similar to the ones in plastic packages. A one-phase heat transfer model has been used to foresee the maximum temperature within the bed and the results agreed fairly well with the experimental ones. Using the model, a bed depth of 7 cm was found to be the limit for the tray bioreactor. The results obtained are very promising for the mass production of conidia of M. anisopliae at lower costs and with more effective control. (AU)

• Processo FAPESP: 06/55641-7 - Desenvolvimento de bioreator rotativo para fermentacao em estado solido.
• Beneficiário: João Cláudio Thoméo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/00996-2 - Desenvolvimento de um biorreator de leito empacotado pseudo-contínuo para fermentação em estado sólido
• Beneficiário: Fernanda Perpétua Casciatori
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular